华中科技大学学报(医学英德文版)
華中科技大學學報(醫學英德文版)
화중과기대학학보(의학영덕문판)
JOURNAL OF TONGJI MEDICAL UNIVERSITY
2003年
4期
369-372
,共4页
廖国宁%李清芬%冯友梅%邓耀祖%李卓娅%龚非力%马丁
廖國寧%李清芬%馮友梅%鄧耀祖%李卓婭%龔非力%馬丁
료국저%리청분%풍우매%산요조%리탁아%공비력%마정
tumor invasion%prostate carcinoma%antisense RNA%u-PAR
To evaluate the specific inhibition of antisense u-PAR on the u-PAR expressions in highly invasive cell subclones and to determine its blocking function in the invasion by those cells, a cDNA fragment of u-PAR obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in antisense orientation. Then the antisense u-PAR recombinant was transfected into highly invasive cell subclones. The u-PAR expression in neo-resistant cells was examined by RT-PCR and immunohistochemical assay. Compared to the control cells, the content of mRNA and protein of u-PAR in transfected cells decreased sharply, and the rate of inhibition was 53 % and 73 %, respectively, indicating that an antisense u-PAR might have played a specific inhibitory role in its expression in the cells, which may provide a good cell model for making further investigation of the inhibitory effects of the antisense u-PAR on invasion in highly invasive cell subclones of human prostate carcinoma.
