CAJ | 학술논문

目的探讨布地奈德(BUD)对哮喘大鼠气道重塑及支气管肺泡灌洗液(BALF)、肺组织中转化生长因子-β1(TGF-β1)的影响,为糖皮质激素在哮喘中的应用提供理论依据.方法健康成年雄性Wistar大鼠60只,随机分为3组.正常对照组、哮喘模型组和BUD治疗组.每组20只.用卵蛋白(OVA)致敏和激发制成哮喘大鼠模型,治疗组应用BUD雾化吸入治疗2周(1 mg/kg,30 min/次,qod),对照组用生理盐水代替.造模完成后,处死大鼠,左肺行支气管肺泡灌洗.右肺行病理学检查.ELISA法检测BALF上清中TGF-β1的含量,免疫组化法检测肺组织中TGF-β1的表达,Masson染色观察肺组织胶原沉积情况,计算机图像分析仪评价呼吸性细支气管平滑肌厚度(μm)及上皮损伤程度评分等气道重塑指标.结果造模后,模型组大鼠BALF上清液及肺组织中TGF-β1的表达较对照组增多(P<0.01),与气道重翅的指标呈显著正相关;治疗组大鼠BALF上清液及肺组织中TGF-β1的表达较模型组减少(P<0.05).气道黏膜上皮损害、气道平滑肌厚度、气道胶原蛋白的沉积较模型组明显减轻.结论哮喘大鼠BALF及肺组织中TGF一β1的表达增多,加重了气道重塑;BUD可以在一定程度上干预哮喘气道重塑,可能是通过减少气道局部TGF-β1的表达来实现的.
목적 탐토포지내덕(BUD)대효천대서기도중소급지기관폐포관세액(BALF)、폐조직중전화생장인자-β1(TGF-β1)적영향,위당피질격소재효천중적응용제공이론의거.방법 건강성년웅성Wistar대서60지,수궤분위3조.정상대조조、효천모형조화BUD치료조.매조20지.용란단백(OVA)치민화격발제성효천대서모형,치료조응용BUD무화흡입치료2주(1 mg/kg,30 min/차,qod),대조조용생리염수대체.조모완성후,처사대서,좌폐행지기관폐포관세.우폐행병이학검사.ELISA법검측BALF상청중TGF-β1적함량,면역조화법검측폐조직중TGF-β1적표체,Masson염색관찰폐조직효원침적정황,계산궤도상분석의평개호흡성세지기관평활기후도(μm)급상피손상정도평분등기도중소지표.결과 조모후,모형조대서BALF상청액급폐조직중TGF-β1적표체교대조조증다(P<0.01),여기도중시적지표정현저정상관;치료조대서BALF상청액급폐조직중TGF-β1적표체교모형조감소(P<0.05).기도점막상피손해、기도평활기후도、기도효원단백적침적교모형조명현감경.결론 효천대서BALF급폐조직중TGF일β1적표체증다,가중료기도중소;BUD가이재일정정도상간예효천기도중소,가능시통과감소기도국부TGF-β1적표체래실현적.
Objectives To explore the effects of budesonide (BUD) on airway remodeling and the levels of transforming growth factor-β1 (TGF-β1) in bronchoalveolar lavage fluid (BALF) and its expression in lung tissue of asthmatic rats. To provide a theoretical basis for the intervention and treatment of asthma. Methods Sixty healthy adult male Wistar rats were randomly divided into 3 groups: control group, asthma model group and BUD treated group, 20 rats in each group. A rat asthma model was established by ovalbumin (OVA) challenging. BUD treated group was treated with inhaled BUD 1 mg/kg in 30 min per serving every other day for two weeks. After OVA challenge finished, the rats were anesthetized and sacrificed for BALF and lung tissue collection. The level of TGF-β1 in BALF was measured by enzyme-linked immunosorbent assay. TGF-β1 expression and collagen deposition in the lung tissue were tested with immunohistochemical determination and Masson stain respectively. The computer image analysis system was used for measuring respiratory bronchiole smooth muscle thickness (μm) and the extent of epithelial damage score and other indicators of airway remodeling. Results Compared with that in the control group, TGF-β1 in BALF and lung tissue of asthma model group increased and were correlated significantly with the indicators of airway remodeling (P < 0.01) ;while that of BUD treated group reduced statistically significant than model group (P < 0.05). Pathology examination on asthma rat airway epithelial tissue showed the thickness of airway smooth muscle, the airway deposition of collagen in BUD treated group reduced obviously than asthma model group. Conclusions The expression of TGF-β1 in the rat asthma airway worsens the airway remodeling. The effects of BUD on ameliorating the progression of airway remodeling may be partially made by reducing the expression of TGF-β1. (J Clin Pediatr,2010,28(2):173-177)