CAJ | 학술논문

目的观察兔悬挂双前肢致死不同组织中PKC、AchE、HSP70的活性变化,探讨其在死亡机制中的作用.方法家兔双前肢悬挂致死亡,取膈肌、肋间肌、心肌、腓肠肌、大脑、脑干、肝、肾、肺等组织,以勒颈、颅脑损伤和疾病死亡相应组织为对照.采用SP法对上述组织中蛋白激酶C(PKC)、乙酰胆碱酯酶(AchE)和热休克蛋白70(HSP70)进行免疫组化染色,观察其活性,并采用多组独立样本的Kruskal-Wallis秩和检验方法,对结果进行统计学分析.结果膈肌、肋间肌和大脑组织细胞质中PKC免疫组化阳性染色,悬挂组较其它组增强(P＜0.05);膈肌、肋间肌、心肌、大脑和肝细胞质及肌组织神经束中AchE免疫组化阳性染色,悬挂组较其它组增强(P＜0.05);大脑、心、肺中HSP70免疫绀化阳性染色,悬挂组较其它组增强(P＜0.05).结论 PKC、AChE、HSP70通过不同机制,在悬挂双上肢体位性窒息死亡中起到一定作用,本文结果可为相关研究及鉴定提供实验依据.
목적 관찰토현괘쌍전지치사불동조직중PKC、AchE、HSP70적활성변화,탐토기재사망궤제중적작용.방법 가토쌍전지현괘치사망,취격기、륵간기、심기、비장기、대뇌、뇌간、간、신、폐등조직,이륵경、로뇌손상화질병사망상응조직위대조.채용SP법대상술조직중단백격매C(PKC)、을선담감지매(AchE)화열휴극단백70(HSP70)진행면역조화염색,관찰기활성,병채용다조독립양본적Kruskal-Wallis질화검험방법,대결과진행통계학분석.결과 격기、륵간기화대뇌조직세포질중PKC면역조화양성염색,현괘조교기타조증강(P＜0.05);격기、륵간기、심기、대뇌화간세포질급기조직신경속중AchE면역조화양성염색,현괘조교기타조증강(P＜0.05);대뇌、심、폐중HSP70면역감화양성염색,현괘조교기타조증강(P＜0.05).결론 PKC、AChE、HSP70통과불동궤제,재현괘쌍상지체위성질식사망중기도일정작용,본문결과가위상관연구급감정제공실험의거.
Objective Investigating activity variance of PKC.AchE and HSP70 in different tissues of rabbits caused by dying from suspension of anterior limbs.in order to study how factors above contribute in the mechanism of death.Methods Suspend rabbit by its anterior limbs until it dies.and coilect tissue from its diaphragmatic muscle,intercostal muscle,myocardium,gastrocnemius,cerebrum,brain stem,liver,kidney and lung.In the control groups,collect same tissues of rabbits dying from strangulation,craniocerebral injury and diseases as contrast.Detect activity of PKC,AchE and HSP70 in tissues of those groups mentioned above by SP stain of immunohistochemistry.and statistically analyze result of detection by rank SUm test of independent samples of multi-groups.Results Compared with data from other control groups,there are more obvious expressions in following tissues of group of suspension:PKC in disphragmatic muscle,intercostal muscles and cerebrum(P＜0.05);AchE in disphragmatic muscles,intercostal muscles,myocardium,cerebrum,liver and nerve tract ofmuscles(P＜0.05);HSP70 in cerebrum,myocardium and lung(P＜0.05).Conclusion Through different mechanisms,PKC,AchE and HSP70 could take some certain contributions in death pmcess of suspension by anterior limbs,and the results above could offer some experimental evidences for related studies and medicolegal investigations.