中国中西医结合杂志
中國中西醫結閤雜誌
중국중서의결합잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN MEDICINE
2009年
12期
1089-1091
,共3页
姜晓晓%李勇%吴永宾%徐建军
薑曉曉%李勇%吳永賓%徐建軍
강효효%리용%오영빈%서건군
葛根素%体外循环%细胞因子
葛根素%體外循環%細胞因子
갈근소%체외순배%세포인자
puerarin%cardiopulmonary bypass%cytokine
目的 探讨葛根素预处理对体外循环术(cardiopulmonary bypass,CPB)围手术期细胞因子的影响及其作用机制.方法 将40例心脏病患者随机分为体外循环手术对照组(20例)和试验组(20例).试验组于术前1周开始用葛根素0.6 g加入5%葡萄糖注射液250 mL中静脉滴注,1次/日;对照组滴注平衡盐,其他处理两组无差别.观察两组在麻醉诱导期、升主动脉阻断后10 min、开放升主动脉后10 min、2 h、12 h共5个时间点肿瘤坏死因子(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin)-6、IL-8、IL-10水平变化.结果 两组患者CPB后上述参数水平开始升高,升主动脉开放后2 h时达到峰值,以后逐渐下降,但12 h仍高于术前(P<0.05),葛根素组TNF-α、IL-6、IL-8水平在各时间点上升幅度均低于对照组(P<0.01或P<0.05),各时间点IL-10水平升高幅度均高于对照组(P<0.01).结论 CPB手术前使用葛根素能有效降低促炎性细胞因子TNF-α、IL-6、IL-8的水平;增加抗炎性细胞因子IL-10的表达,从而减轻CPB术后机体的炎症反应.
目的 探討葛根素預處理對體外循環術(cardiopulmonary bypass,CPB)圍手術期細胞因子的影響及其作用機製.方法 將40例心髒病患者隨機分為體外循環手術對照組(20例)和試驗組(20例).試驗組于術前1週開始用葛根素0.6 g加入5%葡萄糖註射液250 mL中靜脈滴註,1次/日;對照組滴註平衡鹽,其他處理兩組無差彆.觀察兩組在痳醉誘導期、升主動脈阻斷後10 min、開放升主動脈後10 min、2 h、12 h共5箇時間點腫瘤壞死因子(tumor necrosis factor-α,TNF-α)、白細胞介素(interleukin)-6、IL-8、IL-10水平變化.結果 兩組患者CPB後上述參數水平開始升高,升主動脈開放後2 h時達到峰值,以後逐漸下降,但12 h仍高于術前(P<0.05),葛根素組TNF-α、IL-6、IL-8水平在各時間點上升幅度均低于對照組(P<0.01或P<0.05),各時間點IL-10水平升高幅度均高于對照組(P<0.01).結論 CPB手術前使用葛根素能有效降低促炎性細胞因子TNF-α、IL-6、IL-8的水平;增加抗炎性細胞因子IL-10的錶達,從而減輕CPB術後機體的炎癥反應.
목적 탐토갈근소예처리대체외순배술(cardiopulmonary bypass,CPB)위수술기세포인자적영향급기작용궤제.방법 장40례심장병환자수궤분위체외순배수술대조조(20례)화시험조(20례).시험조우술전1주개시용갈근소0.6 g가입5%포도당주사액250 mL중정맥적주,1차/일;대조조적주평형염,기타처리량조무차별.관찰량조재마취유도기、승주동맥조단후10 min、개방승주동맥후10 min、2 h、12 h공5개시간점종류배사인자(tumor necrosis factor-α,TNF-α)、백세포개소(interleukin)-6、IL-8、IL-10수평변화.결과 량조환자CPB후상술삼수수평개시승고,승주동맥개방후2 h시체도봉치,이후축점하강,단12 h잉고우술전(P<0.05),갈근소조TNF-α、IL-6、IL-8수평재각시간점상승폭도균저우대조조(P<0.01혹P<0.05),각시간점IL-10수평승고폭도균고우대조조(P<0.01).결론 CPB수술전사용갈근소능유효강저촉염성세포인자TNF-α、IL-6、IL-8적수평;증가항염성세포인자IL-10적표체,종이감경CPB술후궤체적염증반응.
Objective To study the effect and acting mechanism of puerarin preconditioning (PP) on blood level of cytokines in patients undergoing cardiopulmonary bypass (CPB) in perioperative period. Methads Forty patients with heart diseases scheduled to take surgical operation were randomized into the control group and the PP group equally. They were treated with the same program, excepting that 0.6 g of puerarin was given to the PP group by adding in 5% glucose solution 250 mL for intravenous dripping every day for one week before operation, but to the control group, normal saline was given instead. The levels of tumor necrosis factor-α (TNF-α), interleukin 6, 8 and 10 (IL-6, IL-8, IL-10) in arterial blood were measured at 5 time points in the process of CPB, namely, anesthetic induction (T1), 10 min after the clamp of the ascending aorta (T2), 10 min, 2 h and 12 h after the Clamped aorta is unclamped (T3, T4 and T5). Results All the above-mentioned indexes (TNF-α, IL-6, IL-8 and IL-10) gradually increased after beginning CPB, reached the peak at T4, then lowered gradually but still presented the higher levels at T5 than those at T1 (P<0.05). Comparison between the two groups showed that levels of TNF-α, IL-6 and IL-8 were significantly lower (P<0.05 or P<0.01) and level of IL-10 was higher in the PP group than those in the control group respectively at all the time points (P<0.01). Conclusion Injecting puerarin before CPB could effectively suppress the pro-inflammatory cytokines like TNF-α, IL-6 and IL-8; and enhance the expression of anti-inflammatory cytokines like IL-10, thus to alleviate the inflammatory reaction induced by CPB.
