CAJ | 학술논문

背景:聚乙烯醇具有与人体组织相近的含水量、良好的生物相容性和较高的机械强度,适合作为组织工程基质材料.但如何改善其细胞亲和性,是将其作为组织再生支架材料的关键.目的:制备壳聚糖/聚乙烯醇复合支架,探讨其作为修复人体损伤组织或器官组织工程支架材料的可行性.方法:将固定相对分子质量和醇解度的聚乙烯醇124与固定脱乙酰度的壳聚糖按不同质量配比复合,分别用成膜法、成颗粒法、冷冻干燥法制备壳聚糖/聚乙烯醇复合支架.测定复合膜的透光率、含水率、膨胀率;测定复合颗粒和复合海绵的含水率;并用扫描电镜观察复合支架横截面的形貌.结果与结论:通过改变复合支架中壳聚糖与聚乙烯醇和含量,制备7种不同质量配比的复合支架.成膜法制备的复合支架,其透光率为70%～80%,含水率为121.2%～162.5%,膨胀率为60.3%～133.7%;成颗粒法和冷冻干燥法制备的复合支架,其含水率分别为82.0%～461.2%和280.8%～1939.0%.聚乙烯醇与壳聚糖的质量配比为0.75/0.15时,复合支架的综合性能最好.扫描电镜观察显示,聚乙烯醇与壳聚糖的质量配比为0.75/0.15时,用冷冻干燥法制备的复合支架其内部结构规整,呈蓬松纤维状,有较好的力学性能和较高的含水率.
배경:취을희순구유여인체조직상근적함수량、량호적생물상용성화교고적궤계강도,괄합작위조직공정기질재료.단여하개선기세포친화성,시장기작위조직재생지가재료적관건.목적:제비각취당/취을희순복합지가,탐토기작위수복인체손상조직혹기관조직공정지가재료적가행성.방법:장고정상대분자질량화순해도적취을희순124여고정탈을선도적각취당안불동질량배비복합,분별용성막법、성과립법、냉동간조법제비각취당/취을희순복합지가.측정복합막적투광솔、함수솔、팽창솔;측정복합과립화복합해면적함수솔;병용소묘전경관찰복합지가횡절면적형모.결과여결론:통과개변복합지가중각취당여취을희순화함량,제비7충불동질량배비적복합지가.성막법제비적복합지가,기투광솔위70%～80%,함수솔위121.2%～162.5%,팽창솔위60.3%～133.7%;성과립법화냉동간조법제비적복합지가,기함수솔분별위82.0%～461.2%화280.8%～1939.0%.취을희순여각취당적질량배비위0.75/0.15시,복합지가적종합성능최호.소묘전경관찰현시,취을희순여각취당적질량배비위0.75/0.15시,용냉동간조법제비적복합지가기내부결구규정,정봉송섬유상,유교호적역학성능화교고적함수솔.
BACKGROUND: Polyvinyl alcohol (PVA) has similar water content as human tissues, good biocompatibility and high mechanical strength, thereby being utilized as tissue-engineered matrix material. However, the modification in cellular affinity benefits the function as tissue regeneration scaffold materials. OBJECTIVE: To prepare chitosan (CS) / PVA composite scaffold and explore the feasibility of serving as a scaffold for tissue engineering. METHODS: Scaffolds of CS/PVA was fabricated by blending PVA-124 with various amounts of CS using film forming, particle forming, and free-drying methods. Water content, light transmittance, and swelling ratio were tested. Scanning electron microscope was used to observe the configuration of the cress section. RESULTS AND CONCLUSION: A total of 7 different proportional scaffolds were obtained with different PVA and CS dosage. The composite film showed light transmittance between 70%-80%, high water content between 121.2%-162.5% and swelling ratio between 60.3%-133.7%.Composita particle and sponge obtained water content between 82.0%-461.2% and 280.8%-1 939.0%. Composite scaffold with the amount ratio of PVA 0.75 g/CS 0.15 g exhibited the best performance. Scanning electron microscope showed that at a mass ratio of PVA 0.75 g/CS 0.15 g, the scaffold prepared by freeze-drying method had regular, tluffy inner structure, with good mechanics and high water content.