广西农业科学
廣西農業科學
엄서농업과학
GUANGXI AGRICULTURAL SCIENCES
2010年
12期
1291-1295
,共5页
西葫芦%聚合酶链反应%系统发育分析%帕兰波番茄曲叶病毒
西葫蘆%聚閤酶鏈反應%繫統髮育分析%帕蘭波番茄麯葉病毒
서호호%취합매련반응%계통발육분석%파란파번가곡협병독
C.pepo%PCR%phylogenetic analysis%Tomato leaf curl Palampur virus
2008年对印度北方邦东部Gorakhpur、Kushinagar与Maharajganj等3个地区的西葫芦种植区进行调查,发现西葫芦叶片呈现花叶斑点状、卷曲,植株黄化萎缩.以菜豆金色花叶病毒组外壳蛋白基因特异引物AV1-F、AV1-R对从不同地区收集的西葫芦病斑叶片样品进行PCR分析,获得约800 bp的扩增产物.对从Gorakhpur也区获得的分离物的扩增产物进行克隆和序列分析,测序结果提交GenBank核酸序列数据库;通过核酸BLAST比对和系统发育分析,该分离物与帕兰波番茄曲叶病毒株的同源性最高,达95%,两者遗传关系最近,认为此病毒分离物是帕兰波番茄曲叶病毒株.
2008年對印度北方邦東部Gorakhpur、Kushinagar與Maharajganj等3箇地區的西葫蘆種植區進行調查,髮現西葫蘆葉片呈現花葉斑點狀、捲麯,植株黃化萎縮.以菜豆金色花葉病毒組外殼蛋白基因特異引物AV1-F、AV1-R對從不同地區收集的西葫蘆病斑葉片樣品進行PCR分析,穫得約800 bp的擴增產物.對從Gorakhpur也區穫得的分離物的擴增產物進行剋隆和序列分析,測序結果提交GenBank覈痠序列數據庫;通過覈痠BLAST比對和繫統髮育分析,該分離物與帕蘭波番茄麯葉病毒株的同源性最高,達95%,兩者遺傳關繫最近,認為此病毒分離物是帕蘭波番茄麯葉病毒株.
2008년대인도북방방동부Gorakhpur、Kushinagar여Maharajganj등3개지구적서호호충식구진행조사,발현서호호협편정현화협반점상、권곡,식주황화위축.이채두금색화협병독조외각단백기인특이인물AV1-F、AV1-R대종불동지구수집적서호호병반협편양품진행PCR분석,획득약800 bp적확증산물.대종Gorakhpur야구획득적분리물적확증산물진행극륭화서렬분석,측서결과제교GenBank핵산서렬수거고;통과핵산BLAST비대화계통발육분석,해분리물여파란파번가곡협병독주적동원성최고,체95%,량자유전관계최근,인위차병독분리물시파란파번가곡협병독주.
In a survey of cucurbit growing areas of Gorakhpur,Kushinagar and Maharajganj districts (Eastern U.P.,India)during 2008.Cucurbita pepo plants were found infected with mosaic mottling,severe mosaic,leaf curling,and yellowing and stunting of whole plants.PCR assay,using begomovirus coat protein gene specific primers (AV1-F & AVl-R),resulted in amplification of ～800 bp product in symptomatic leaf samples of C.pepo collected from all the locations.The amplified product of Gorakhpur isolate was cloned and sequenced,and data was submitted in GenBank.Nucleotide BLAST and phylogenetic analyses of the virus isolate showed highest (95%) sequence identity and a close relationships with the isolates of Tomato leaf curl Palampur virus (ToLCPV),suggesting the virus isolate to be the strain of ToLCPV.
