CAJ | 학술논문

背景:骨科手术后易出现深静脉血栓形成,但目前临床上对此尚缺乏有效预测诊断手段,组织蛋白酶可能是血栓形成的有效生物标记物.目的:观察大鼠深静脉血栓形成前后组织蛋白酶B和组织蛋白酶 C在血细胞中的表达变化情况,探讨二者作为深静脉血栓形成早期诊断候选分子标志物的可行性.方法:将100只SD大鼠随机分为正常对照组和模型组,模型组采用血管钳夹股静脉+双后肢固定制动的方式建立大鼠创伤性深静脉血栓模型,根据观察时间点和血栓形成情况分为血栓形成前组、血栓形成高峰期组和血栓不形成组,提取各组血液RNA并反转录为cDNA,应用实时荧光定量PCR检测组织蛋白酶 B和组织蛋白酶 C在血细胞中的表达变化情况.结果与结论:血栓形成高峰期组大鼠血细胞中组织蛋白酶B,C 表达明显,血栓形成前组和血栓不形成组大鼠血细胞中组织蛋白酶B,C表达于正常对照组大鼠为无明显差异.提示组织蛋白酶B和组织蛋白酶C与深静脉血栓形成密切相关,可作为深静脉血栓形成早期诊断的候选分子标志物.
배경:골과수술후역출현심정맥혈전형성,단목전림상상대차상결핍유효예측진단수단,조직단백매가능시혈전형성적유효생물표기물.목적:관찰대서심정맥혈전형성전후조직단백매B화조직단백매 C재혈세포중적표체변화정황,탐토이자작위심정맥혈전형성조기진단후선분자표지물적가행성.방법:장100지SD대서수궤분위정상대조조화모형조,모형조채용혈관겸협고정맥+쌍후지고정제동적방식건립대서창상성심정맥혈전모형,근거관찰시간점화혈전형성정황분위혈전형성전조、혈전형성고봉기조화혈전불형성조,제취각조혈액RNA병반전록위cDNA,응용실시형광정량PCR검측조직단백매 B화조직단백매 C재혈세포중적표체변화정황.결과여결론:혈전형성고봉기조대서혈세포중조직단백매B,C 표체명현,혈전형성전조화혈전불형성조대서혈세포중조직단백매B,C표체우정상대조조대서위무명현차이.제시조직단백매B화조직단백매C여심정맥혈전형성밀절상관,가작위심정맥혈전형성조기진단적후선분자표지물.
BACKGROUND: Deep venous thrombosis (DVT) always occurs after orthopedic surgery. At present, clinical diagnosis of DVT has been lack of an effective measuring means for a long time. Cathepsin may be an effective biological marker of DVT. OBJECTIVE: To study the expression change of cathepsin B and cathepsin C in the rat blood cells before and after DVT and to investigate the feasibility of cathepsin B and cathepsin C as candidate molecular markers for early diagnosis of DVT. METHODS: Totally 100 Sprague Dawley rats were randomly divided into normal control group (n=10) and model group (n=90). Rat traumatic deep vein thrombosis models were established by clamping the femoral vein and fixing the bilateral hind limbs. According to observation time points and the different situations of thrombosis, rat models were assigned to three subgroups: pre-thrombosis, intra-thrombosis, and non-thrombosis. Blood RNA of each group was extracted and reverse transcribed into cDNA. The expression of cathepsin B and cathepsin C in blood cells was detected using real-time fluorescence quantitative PCR. RESULTS AND CONCLUSUON: Expression of cathepsin B and cathepsin C in the blood cells was obviously expressed in the intra-thrombosis subgroup. There was no significant difference in cathepsin B and cathepsin C expression between pre-thrombosis, non-thrombosis groups and normal control group. These findings suggest that cathepsin B and cathepsin C are closely related to DVP and they can be used as the candidate molecular markers for early diagnosis of DVT.