CAJ | 학술논문

目的比较含砷的复方黄黛片、雄黄与砷酸钠的毒性.方法小鼠一次性灌胃给药,8h后检测血生化及肝肾病理变化；测定肝肾组织中的砷蓄积量及砷毒性敏感基因的表达.结果砷酸钠组肝肾砷蓄积量显著增加,并伴肝肾功能和病理损伤,MT-1、MT-2、HO-1和IL-1β基因表达明显上调.复方黄黛片组和雄黄组的肝肾功能及病理检查与正常对照组相似,未见异常.二者砷蓄积量仅为砷酸钠组的1/25;上述基因表达仅轻微升高.结论复方黄黛片与雄黄的急性毒性远小于砷酸钠,不宜单用总砷含量评价含砷中成药的毒性.
목적 비교함신적복방황대편、웅황여신산납적독성.방법 소서일차성관위급약,8h후검측혈생화급간신병리변화；측정간신조직중적신축적량급신독성민감기인적표체.결과 신산납조간신신축적량현저증가,병반간신공능화병리손상,MT-1、MT-2、HO-1화IL-1β기인표체명현상조.복방황대편조화웅황조적간신공능급병리검사여정상대조조상사,미견이상.이자신축적량부위신산납조적1/25;상술기인표체부경미승고.결론 복방황대편여웅황적급성독성원소우신산납,불의단용총신함량평개함신중성약적독성.
Objective Huang-Dai Pian (HDP) is a realgar ( As4S4 )-containing Chinese medicine for hematological malignancies.Realgar is the main component but is often taken as arsenate for risk assessment.To evaluate true risk of realgar and HDP,acute toxicity was compared with arsenate in mice.Methods Mice were orally given HDP and equivalent dose of realgar(20 mg As/kg),sodium arsenate( 10 mg As/kg),and acute toxicity and tissue arsenic content were determined.Results Arsenate increased serum ALT,AST and BUN levels,which is indicative of liver and kidney injury.Histopathology showed severe damage in arsenate-treated murine liver and kidney,while in HDP and realgar-treated animals,these lesions were mild or absent.And there was no elevation in serum biomarkers.Hepatic and renal arsenic contents were dramatically increased to 3 560 and 4 570 ng/g,respectively following arsenate administration,but only increased to 125 and 175 ng/g after HDP or realgar intake.Expressions of arsenic toxicity sensitive genes,namely metallothionein( MT- 1 and MT- 2 ),heme oxygenase- 1 ( HO- 1 )and interleukin-1β( IL-1β)were significantly increased by arsenate,but only slightly increased by HDP and realgar.Conclusion HDP and realgar are much less acutely toxic than sodium arsenate.It may be inappropriate to use total arsenic content for evaluating the safety of realgar-containing traditional medicine such as HDP.