国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2011年
5期
294-298
,共5页
吴海波%沃恩康%尤金彪%王怡婷%王巧刚%吴南屏%郭潮潭
吳海波%沃恩康%尤金彪%王怡婷%王巧剛%吳南屏%郭潮潭
오해파%옥은강%우금표%왕이정%왕교강%오남병%곽조담
鸡贫血病毒%vp3基因%克隆%分子特征
鷄貧血病毒%vp3基因%剋隆%分子特徵
계빈혈병독%vp3기인%극륭%분자특정
Chicken anemia virus%vp3 gene%Cloning%Molecular characteristics
目的 获得来源于浙江地区鸡组织中的鸡贫血病毒(CA)vp3基因并分析其变异情况.方法 根据GenBank登录的vp3序列设计特异引物,利用PCR方法从鸡组织中获得7个CAV的vp3基因克隆,将其克隆到载体进行测序.结果 40份鸡血清中共检出CAV阳性16份,阳性率40.0%.序列测定结果表明,vp3基因长366 bp,编码121个氨基酸,与GenBank登录的其他毒株vp3基因的核苷酸序列同源性为97.0% ~ 100.0%,氨基酸序列同源性为95.0% ~ 100.0%.结论 通过PCR方法扩增获得了CAV vp3基因,vp3基因及VP3蛋白总体上非常保守.
目的 穫得來源于浙江地區鷄組織中的鷄貧血病毒(CA)vp3基因併分析其變異情況.方法 根據GenBank登錄的vp3序列設計特異引物,利用PCR方法從鷄組織中穫得7箇CAV的vp3基因剋隆,將其剋隆到載體進行測序.結果 40份鷄血清中共檢齣CAV暘性16份,暘性率40.0%.序列測定結果錶明,vp3基因長366 bp,編碼121箇氨基痠,與GenBank登錄的其他毒株vp3基因的覈苷痠序列同源性為97.0% ~ 100.0%,氨基痠序列同源性為95.0% ~ 100.0%.結論 通過PCR方法擴增穫得瞭CAV vp3基因,vp3基因及VP3蛋白總體上非常保守.
목적 획득래원우절강지구계조직중적계빈혈병독(CA)vp3기인병분석기변이정황.방법 근거GenBank등록적vp3서렬설계특이인물,이용PCR방법종계조직중획득7개CAV적vp3기인극륭,장기극륭도재체진행측서.결과 40빈계혈청중공검출CAV양성16빈,양성솔40.0%.서렬측정결과표명,vp3기인장366 bp,편마121개안기산,여GenBank등록적기타독주vp3기인적핵감산서렬동원성위97.0% ~ 100.0%,안기산서렬동원성위95.0% ~ 100.0%.결론 통과PCR방법확증획득료CAV vp3기인,vp3기인급VP3단백총체상비상보수.
Objective To obtain the chicken anemia virus(CAV) vp3 gene from Zhejiang region chicken and analyze its variation.Methods The specific primers were designed according to the published sequences in GenBank.By PCR,7 clones of CAV vp3 gene were obtained from CAV-infected chicken.Results 16 CAV-positive chicken serum were detected in 40 samples,and the positive rate was 40.0%.Sequencing results showed that vp3 gene consisted of 366 bp,coded 121 amino acids,and shared 97.0%-100.0% nucleotide homology and 95.0%-100.0% protein homology with that of the other strains submitted to GenBank.Conclusions CAV vp3 gene is obtained by PCR,vp3 gene and VP3 protein are very conservative.