CAJ | 학술논문

目的探讨亚慢性饮水砷暴露小鼠血和肝中砷形态的分布,评价砷对血和肝中还原型谷胱甘肽(GSH)水平的影响.方法小鼠以自由饮水方式饮用含砷量为0(对照)、25、50、100mg/L的水溶液,连续染砷6周.采用氢化物发生-超低温捕集-原子吸收分光光度法,检测小鼠血和肝中无机砷(iAs)、一甲基砷酸(MMA)和二甲基砷酸(DMA)的水平,计算甲基化率.采用二硫双硝基苯甲酸(DTNB)法测定全血和肝组织中GSH的水平.结果小鼠血和肝中iAs、MMA和DMA水平随染砷剂量的增加而升高.各染砷组小鼠血和肝中的砷一甲基化率(PMI)和二甲基化率(SMI)与对照组比较差异均有统计学意义(P＜0.05),其中50 mg/L染砷组小鼠肝中SMI[(50.45±2.94)%]明显高于25 mg/L组[(41.68±7.09)%]和100 mg/L组[(41.19±8.87)%],组间比较差异有统计学意义(P＜0.05).各染砷组小鼠血和肝中砷形态的构成比(iAs:MMA:DMA)分别为2:3:5和4:3:3.有机砷(MMA+DMA)的水平分别约占总砷的80%和60%.各染砷组小鼠血和肝中GSH水平随染砷剂量的增加而下降,与对照组比较差异均有统计学意义(P＜0.05),但各染砷组之间的小鼠血和肝中GSH水平比较差异均无统计学意义(P＞0.05).结论当砷暴露水平达到一定程度后,肝脏对iAs的甲基化代谢能力可达到饱和.血中砷形态变化不同于肝组织,提示机体内的其他组织器官也可能具有砷甲基化代谢能力.血和肝中GSH水平是反映砷毒性的较好指标.
목적 탐토아만성음수신폭로소서혈화간중신형태적분포,평개신대혈화간중환원형곡광감태(GSH)수평적영향.방법 소서이자유음수방식음용함신량위0(대조)、25、50、100mg/L적수용액,련속염신6주.채용경화물발생-초저온포집-원자흡수분광광도법,검측소서혈화간중무궤신(iAs)、일갑기신산(MMA)화이갑기신산(DMA)적수평,계산갑기화솔.채용이류쌍초기분갑산(DTNB)법측정전혈화간조직중GSH적수평.결과 소서혈화간중iAs、MMA화DMA수평수염신제량적증가이승고.각염신조소서혈화간중적신일갑기화솔(PMI)화이갑기화솔(SMI)여대조조비교차이균유통계학의의(P＜0.05),기중50 mg/L염신조소서간중SMI[(50.45±2.94)%]명현고우25 mg/L조[(41.68±7.09)%]화100 mg/L조[(41.19±8.87)%],조간비교차이유통계학의의(P＜0.05).각염신조소서혈화간중신형태적구성비(iAs:MMA:DMA)분별위2:3:5화4:3:3.유궤신(MMA+DMA)적수평분별약점총신적80%화60%.각염신조소서혈화간중GSH수평수염신제량적증가이하강,여대조조비교차이균유통계학의의(P＜0.05),단각염신조지간적소서혈화간중GSH수평비교차이균무통계학의의(P＞0.05).결론 당신폭로수평체도일정정도후,간장대iAs적갑기화대사능력가체도포화.혈중신형태변화불동우간조직,제시궤체내적기타조직기관야가능구유신갑기화대사능력.혈화간중GSH수평시반영신독성적교호지표.
Objective To explore the distribution of arsenic speciafion and to estimate the effect of arsenic on glutathione(GSH)levels in the blood and liver of mice exposed to different concentrations of inorganic AsⅢ through drinking water.Methods Mice drank water containing arsenite at concentrations of iAsⅢ of 0(contr01),25,50,100 ms/L for 6 weeks.Blood and liver were sampled to asses$the levels of inorganic arsenic(iAs),monomethylarsenic acid(MMA),dimethylarsenic acid(DMA)by the method of hydride generation trapping and ultra-hypothermia coupled with atomic absorption spectrometry,and the level of GSH by the method of 5,5'-Dithio-bis (2-Nitrobenzoic acid).Results Leveh of iAs.MMA and DMA in blood and in liver increased along with the increase of iAs concentrations in drinking water.Primary methylated index(PMI)and secondary methylation index (SMI)of liver and blood were significantly higher in exposed groups than those in control group(P＜0.05).SMI of liver in 50 mg/L exposed group[(50.45±2.94)%]was significantly higher than those in 25 mg/L and 100 mg/Lgroups[(41.68±7.09)%and(41.19±8.87)%,respectively],the difference being statistically significant(P＜0.05).The ratio of iAs.MMA and DMA in blood and liver in exposed group were 2:3:5 and 4:3:3,the percentage of level of organic arsenic(MMA+DMA)were 80%and 60%.GSH in blood and liver in exposed group decreased along with iAs concentrations in drinking water and had significant differences compared with those in control group (P＜0.05).However,levels of GSH in liver and blood did not differ significantly between exposed groups and control group(P＞0.05).Conclusions Membolism of iAs in liver is maximized when the iAs concentrations in drinking water increases to a certain level.However,the percentage of arsenic speciation in blood is different from that in liver,suggesting that other organs and tissues may be capable of methylation of inorganic arsenic.The level of GSH in liver and blood in mice is a good mark tO reflect the toxicity of arsenic.