CAJ | 학술논문

目的探讨细粒棘球蚴重组铁蛋白疫苗对原头蚴攻击感染的保护性效果.方法 ICR小鼠随机分为3组(每组12只):A组(实验组)、B组(佐剂+PBS组)、C组(空白对照组),A组、B组共免疫3次,每次间隔2周,C组不做任何处理;免疫接种后8周3组均以1100只原头蚴攻击感染,每次免疫前及攻击感染前断尾取血,留取血清;攻击感染后7个月,剖杀小鼠,查检腹腔内包囊数进行统计学分析.结果实验组与佐剂对照组、空白对照组比较,其结果均具有统计学意义(P＜0.05),经计算保护力分别为:73%,85%;佐剂组与空白对照组比较,其结果亦具有统计学意义(P＜0.05),经计算保护力为:42%.结论细粒棘球蚴重组铁蛋白可诱导小鼠产生部分免疫保护力,可作为"鸡尾酒"疫苗的组成部分进一步研究.
목적 탐토세립극구유중조철단백역묘대원두유공격감염적보호성효과.방법 ICR소서수궤분위3조(매조12지):A조(실험조)、B조(좌제+PBS조)、C조(공백대조조),A조、B조공면역3차,매차간격2주,C조불주임하처리;면역접충후8주3조균이1100지원두유공격감염,매차면역전급공격감염전단미취혈,류취혈청;공격감염후7개월,부살소서,사검복강내포낭수진행통계학분석.결과 실험조여좌제대조조、공백대조조비교,기결과균구유통계학의의(P＜0.05),경계산보호력분별위:73%,85%;좌제조여공백대조조비교,기결과역구유통계학의의(P＜0.05),경계산보호력위:42%.결론 세립극구유중조철단백가유도소서산생부분면역보호력,가작위"계미주"역묘적조성부분진일보연구.
Objective To investigate protection by immunization with recombinant Ferritin vaccine of Echinococcus granulosus against protoscolices.Methods ICR mice were randomized into 3groups of 12 mice in each.The mice in group A and B were immunized three times with an interval of two weeks and those in group C did nothing.The animals in all the 3 groups were challenged with 1100 protoscolices intraperitoneally on the 8th week.Serum samples were collected before each inoculation and challenge injection.Seven months later, all the mice were killed and examinated for hydatid cysts.Result The number of cysts was significantly lower in the group A than in group B and C (P＜0.05).The levels of protection afforded were found to be 73% and 85%, respectively.Meanwhile,the number of cysts was markedly lower in group B than in group C(P＜0.05).The rate of protection afforded was 42%.Conclusion Recombinant Ferritin vaccine of Echinococcus granulosus shows partial immune protection.Therefore, it might be a suitable candidate for cocktail vaccine study in the future.