CAJ | 학술논문

目的研究内源性雌激素对糖尿病小鼠血糖、血清胰岛素水平和抗氧化能力的影响,探讨内源性雌激素对胰岛细胞和胰岛素敏感性可能的保护作用.方法小鼠按体重随机分为4组,假手术组(Sham)、去卵巢手术组(Ovx)、假手术注射链脲佐菌素(streptozotocin,STZ)组(Sham+ STZ)、去卵巢手术注射STZ组(Ovx+STZ).切除小鼠两侧卵巢,腹腔注射STZ制糖尿病小鼠模型.跟踪测定体重、血糖,进行糖耐量试验.取血制血清,取出胰腺于4％多聚甲醛中固定.测定血清中胰岛素浓度,丙二醛(MDA)、总抗氧化力( T-AOC),所取胰腺做病理切片,观察胰岛细胞的变化.结果 Ovx组血糖和MDA值明显高于Sham组,总抗氧化力低于Sham组；Ovx+STZ血糖和MDA值高于Sham+STZ组,胰岛素水平和T-AOC要低于Sham+STZ组.结论内源性雌激素对糖尿病小鼠具有一定保护作用.
목적 연구내원성자격소대당뇨병소서혈당、혈청이도소수평화항양화능력적영향,탐토내원성자격소대이도세포화이도소민감성가능적보호작용.방법 소서안체중수궤분위4조,가수술조(Sham)、거란소수술조(Ovx)、가수술주사련뇨좌균소(streptozotocin,STZ)조(Sham+ STZ)、거란소수술주사STZ조(Ovx+STZ).절제소서량측란소,복강주사STZ제당뇨병소서모형.근종측정체중、혈당,진행당내량시험.취혈제혈청,취출이선우4％다취갑철중고정.측정혈청중이도소농도,병이철(MDA)、총항양화력( T-AOC),소취이선주병리절편,관찰이도세포적변화.결과 Ovx조혈당화MDA치명현고우Sham조,총항양화력저우Sham조；Ovx+STZ혈당화MDA치고우Sham+STZ조,이도소수평화T-AOC요저우Sham+STZ조.결론 내원성자격소대당뇨병소서구유일정보호작용.
Objective To investigate the effects of endogenous estrogen on blood glucose level,serum insulin level,and plasma total antioxidant capacity in streptozotocin-induced diabetic female mice,and to explore possible protective effects of estrogen on pancreatic islet cells.Methods Female mice were randomly according to body weight divided into four groups:( 1 ) Sham( Sham operation and vehicle administration) ; ( 2 ) Ovx( ovariectomy and vebicle administration ) ; ( 3 ) Sham + STZ ( Sham operation and STZ administration ) ; and ( 4 ) Ovx + STZ ( ovariectomy and STZ administration).The diabetic mice were induced by intraperitoneal injection of streptozotocin (STZ,50 mg/kg ).Blood glucose levels were measured once a week.Results The blood glucose level and malondialdehyde of Ovx group were higher than that in Sham group,while total anti-oxidant capacity ( T-AOC ) was lower than those in Sham group.the blood glucose level and MDA of Ovx+ STZ group were higher than those in Sham +STZ group,while T-AOC and serum insulin level were lower than those in Sham + STZ group.Conclusions Endogenous estrogen may have some protective effects on pancreatic islet function from streptozotocin-induced diabetes mellitus in female mice.