分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001年
1期
11-14
,共4页
基体辅助激光解吸电离%飞行时间质谱%定量%人能皂甙Rg3
基體輔助激光解吸電離%飛行時間質譜%定量%人能皂甙Rg3
기체보조격광해흡전리%비행시간질보%정량%인능조대Rg3
考察了基体辅助激光解吸电离时间飞行质谱用于人参皂甙Rg3定量分析时内标的选择。加入棉子糖作为内标时，Rg3的定量标准曲线的回归系数R2=0.938，平均相对误差为28.6%；加入性质相近的芦丁后，Rg3的定量标准曲线的回归系数R2＝0.993，平均相对误差为7.5%。分辨率的提高以及采用Rg3和内标物的质谱峰的相对面积来代表Rg3的浓度可以使定量的重现性明显改善。
攷察瞭基體輔助激光解吸電離時間飛行質譜用于人參皂甙Rg3定量分析時內標的選擇。加入棉子糖作為內標時，Rg3的定量標準麯線的迴歸繫數R2=0.938，平均相對誤差為28.6%；加入性質相近的蘆丁後，Rg3的定量標準麯線的迴歸繫數R2＝0.993，平均相對誤差為7.5%。分辨率的提高以及採用Rg3和內標物的質譜峰的相對麵積來代錶Rg3的濃度可以使定量的重現性明顯改善。
고찰료기체보조격광해흡전리시간비행질보용우인삼조대Rg3정량분석시내표적선택。가입면자당작위내표시，Rg3적정량표준곡선적회귀계수R2=0.938，평균상대오차위28.6%；가입성질상근적호정후，Rg3적정량표준곡선적회귀계수R2＝0.993，평균상대오차위7.5%。분변솔적제고이급채용Rg3화내표물적질보봉적상대면적래대표Rg3적농도가이사정량적중현성명현개선。
The quantitation of ginsenoside Rg3 by matrix-assisted laserdesorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was investigated. The selection of internal reference was described. The linear regression coefficient of calibration curve was 0.938 and the average relative error was 28.6% if raffinose was added as internal reference, while the linear regression coefficient of calibration curve was 0.993 and the average relative error was 7.5% if rutin was added as internal reference. In addition, the precision was improved when resolution was enhanced and relative peak integral stood for concentration of Rg3.
