CAJ | 학술논문

试验研究了不同培养基对秦党叶片及茎段培养的效应.结果表明.在光照强度1 600lx,温度25℃的条件下,利用组织培养技术快速繁殖秦党时,适宜的外植体为幼苗的茎段.茎段培养时,诱导愈伤组织并促进分化的适宜培养基组分为:MS+2,4-D 0.2 mg/L+蔗糖20 g/L+琼脂8g/L.仅促进芽生长适宜的培养基组分为:MS+BA2.0 mg/L+IAA1.01mg/L+蔗糖20g/L+琼脂8g/L.仅诱导愈伤组织适宜的培养基组分为:MS+BA1.0 mg/L+IAA1.0 mg/L+蔗糖20g/L+琼脂8 g/L.
시험연구료불동배양기대진당협편급경단배양적효응.결과표명.재광조강도1 600lx,온도25℃적조건하,이용조직배양기술쾌속번식진당시,괄의적외식체위유묘적경단.경단배양시,유도유상조직병촉진분화적괄의배양기조분위:MS+2,4-D 0.2 mg/L+자당20 g/L+경지8g/L.부촉진아생장괄의적배양기조분위:MS+BA2.0 mg/L+IAA1.01mg/L+자당20g/L+경지8g/L.부유도유상조직괄의적배양기조분위:MS+BA1.0 mg/L+IAA1.0 mg/L+자당20g/L+경지8 g/L.
It had studied the effect of different culture medium to the culture of Codonopsis tsinlingensis'lamina and stem,and the results showed that when the intensity of illumination was 1 600 lx and the temperature was 25℃,the suitable explant was the young stem when using tissue culture technology to breed Codonopsis tsinlingensis quickly.The culture medium that could inducement callus and accelerate differentiation was MS+2,4-D 0.2 mg/L+sucrose 20 g/L+agar 8 g/L.The culture medium that only could accelerate bud growth was MS+BA2.0 mg/L+IAA1.0 mg/L+sucrose 20 g/L+agar 8 g/L.The culture medium that only could inducement callus was MS+BA1.0 mg/L+IAA1.0 mg/L+sucrose 20 g/L+agar 8 g/L.