CAJ | 학술논문

为了解玉米温敏自交不亲和材料HE97的表达机制,构建了该材料自交亲和与不亲和阶段花丝的正向与反向抑制差减杂交文库,并对其中的219个正向克隆和52个反向克隆进行了测序分析,分别获得了49个和20个Unique ESTs,涉及到代谢、光合作用、信号转导、物质运输、蛋白质合成加工、防御反应、细胞的发育和衰老、基因表达等重要生命过程中的12种不同功能类型.通过电子克隆获得了包含1090 bp的烯脂酰辅酶A水合酶基因,并对其蛋白质功能进行了分析.
위료해옥미온민자교불친화재료HE97적표체궤제,구건료해재료자교친화여불친화계단화사적정향여반향억제차감잡교문고,병대기중적219개정향극륭화52개반향극륭진행료측서분석,분별획득료49개화20개Unique ESTs,섭급도대사、광합작용、신호전도、물질운수、단백질합성가공、방어반응、세포적발육화쇠로、기인표체등중요생명과정중적12충불동공능류형.통과전자극륭획득료포함1090 bp적희지선보매A수합매기인,병대기단백질공능진행료분석.
To understand the express.ion mechanism of a thermo-sensitive genic self-incompatible line HE97 in maize, the forward and reverse library was constructed by means of suppression subtractive hybridization (SSH) method, using the different silks as the material,taken from the self-compatible and the self-incompatible stages. After 219 forward clones and 52 revise clones were sequenced, 49 unique expressed sequence tads (ESTs) in forward library and 20 unique ESTs in reverse library were obtained by clustering and blast analysis. The ESTs were sorted into 12 different functional categories, involving in the process of metabolism, transcription, signal transduction, cell growth/division, trans-porters , and diseases defence. One enoyl CoA hydratase gene with 1 090 bp was cloned by silicon clo-ning, and the protein function was analyzed.