CAJ | 학술논문

蛋白质组学的主要目的是鉴定出生物体内的蛋白质的种类和数量.为达到这个目的,人们开发了多种蛋白质鉴定算法,包括数据库搜索方法、De Novo方法、PST(肽段序列标签)方法和质谱数据库方法.首先介绍了质谱仪中肽段断裂机理的研究,以及相关的质谱鉴定方法,然后综述了当前常用的蛋白质鉴定方法,分析了这些方法的优缺点,最后提出了自己的见解和展望.
단백질조학적주요목적시감정출생물체내적단백질적충류화수량.위체도저개목적,인문개발료다충단백질감정산법,포괄수거고수색방법、De Novo방법、PST(태단서렬표첨)방법화질보수거고방법.수선개소료질보의중태단단렬궤리적연구,이급상관적질보감정방법,연후종술료당전상용적단백질감정방법,분석료저사방법적우결점,최후제출료자기적견해화전망.
One of the main objectives of proteomics is to identify and quantify proteins in a tissue or a cell. Tandem mass spectrum has been proved to be a powerful tool to achieve this goal. This paper presents a review on the protein identification approaches based on tandem mass spectrometry, including database searching methods, De Novo methods, PST (peptide sequence tag) methods, and spectra library methods. It also introduces the research on peptide fragmentation principle, amino acids neutral losses, and peptide identification. Finally, the viewpoints on the development of mass spectrum techniques are described.