药学服务与研究
藥學服務與研究
약학복무여연구
PHARMACEUTICAL CARE AND RESEARCH
2010年
1期
21-23
,共3页
陆松伟%朱全刚%高习清%陈方剑
陸鬆偉%硃全剛%高習清%陳方劍
륙송위%주전강%고습청%진방검
川利明涂剂%盐酸川芎嗪%盐酸利多卡因%盐酸苯海拉明%色谱法,高压液相
川利明塗劑%鹽痠川芎嗪%鹽痠利多卡因%鹽痠苯海拉明%色譜法,高壓液相
천리명도제%염산천궁진%염산리다잡인%염산분해랍명%색보법,고압액상
Chuanliming paint%ligustrazine hydrochloride%lidocaine hydrochloride%diphenhydramine hydrochloride%chromatography,high pressure liquid
目的:建立同时测定川利明涂剂中盐酸川芎嗪、盐酸利多卡因和盐酸苯海拉明含量的RP-HPLC法.方法:色谱柱为Kromasil-C_(18) 柱(150 mm×4.6 mm,5 μm);流动相为乙腈-0.02 mol/L磷酸二氢钠-三乙胺(37:63:0.15),用磷酸调节pH至3.15,内含0.015 mol/L十二烷基硫酸钠;检测波长220 nm;流速1.0 ml/min.结果:盐酸川芎嗪、盐酸利多卡因和盐酸苯海拉明分别在5～80 μg/ml(r=0.999 8)、5.72～91.52 μg/ml(r=0.999 9)、5.26～84.16 μg/ml(r=0.999 6)范围内线性良好,平均回收率分别为(102.82±0.96)%、(103.21±0.91)%和(103.51±0.68)%(n=3),日内、日间精密度符合要求.结论:该方法简便、准确,重复性好,适用于川利明涂剂的质量控制.
目的:建立同時測定川利明塗劑中鹽痠川芎嗪、鹽痠利多卡因和鹽痠苯海拉明含量的RP-HPLC法.方法:色譜柱為Kromasil-C_(18) 柱(150 mm×4.6 mm,5 μm);流動相為乙腈-0.02 mol/L燐痠二氫鈉-三乙胺(37:63:0.15),用燐痠調節pH至3.15,內含0.015 mol/L十二烷基硫痠鈉;檢測波長220 nm;流速1.0 ml/min.結果:鹽痠川芎嗪、鹽痠利多卡因和鹽痠苯海拉明分彆在5～80 μg/ml(r=0.999 8)、5.72～91.52 μg/ml(r=0.999 9)、5.26～84.16 μg/ml(r=0.999 6)範圍內線性良好,平均迴收率分彆為(102.82±0.96)%、(103.21±0.91)%和(103.51±0.68)%(n=3),日內、日間精密度符閤要求.結論:該方法簡便、準確,重複性好,適用于川利明塗劑的質量控製.
목적:건립동시측정천리명도제중염산천궁진、염산리다잡인화염산분해랍명함량적RP-HPLC법.방법:색보주위Kromasil-C_(18) 주(150 mm×4.6 mm,5 μm);류동상위을정-0.02 mol/L린산이경납-삼을알(37:63:0.15),용린산조절pH지3.15,내함0.015 mol/L십이완기류산납;검측파장220 nm;류속1.0 ml/min.결과:염산천궁진、염산리다잡인화염산분해랍명분별재5～80 μg/ml(r=0.999 8)、5.72～91.52 μg/ml(r=0.999 9)、5.26～84.16 μg/ml(r=0.999 6)범위내선성량호,평균회수솔분별위(102.82±0.96)%、(103.21±0.91)%화(103.51±0.68)%(n=3),일내、일간정밀도부합요구.결론:해방법간편、준학,중복성호,괄용우천리명도제적질량공제.
Objective: To establish a RP-HPLC method for simultaneous determination of ligustrazine hydrochloride, lidocaine hydrochloride and diphenhydramine hydrochloride in Chuanliming paint. Methods: The separation was performed on a Kromasil C_(18) column (150 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0.02 mol/L sodium dihydrogen phosphate-triethylamine (37:63:0.15,pH adjusted to 3.15 with phosphoric acid containing 0.015 mol/L sodium dodecyl sulfate).The detection wavelength was 220 nm.The flow rate was 1.0 ml/min. Results:The standard curves were linear in the range of 5-80 μg/ml for ligustrazine hydrochloride (r=0.999 8), 5.72-91.52 μg/ml for lidocaine hydrochloride (r=0.999 9) and 5.26-84.16 μg/ml (r=0.999 6) for diphenhydramine hydrochloride, respectively. The average recovery rates of ligustrazine hydrochloride, lidocaine hydrochloride and diphenhydramine hydrochloride were (102.82±0.96)%,(103.21±0.91)% and (103.51±0.68)%(n=3), respectively.The intra- and inter-day RSD fulfilled the requirements of pharmcopeia. Conclusion:The method is simple and accurate with a good reproducibility, and can be used for quality control of Chuanliming paint.
