CAJ | 학술논문

目的研究并殖吸虫感染者Th2免疫应答产生的细胞因子IL-5、IL-4、IL-13、胸腺基质淋巴细胞生成素(thymic stromal lymphopoietin,TSLP)的水平,筛选出可以用于临床诊断并殖吸虫病的潜在细胞因子标志物.方法收集贵州省本地并殖吸虫病患者成年组(年龄＞18周岁)及未成年组患者(年龄≤18周岁)的血清样本各12例,以健康人血清4例为对照,ELISA检测血清中的IL-5、IL-4、IL-13、TSLP等4种细胞因子的浓度,各病例组及对照组间采用独立样本t检验分析,同时收集患者的流行病学信息.结果成年组IL-5、IL-4、IL-13、TSLP分泌浓度分别为(19.50±5.57)pg/ml、(0.95±0.19)pg/ml、(475.30±629.81) pg/ml、(16 676.67±7 169.29) pg/ml；未成年组分别为(23.04±3.37)pg/ml、(1.05 ±0.19) pg/ml、(422.84±539.48) pg/ml、(16 242.50±6 230.81) pg/ml；对照组分别为(4.45±0.84) pg/ml、(0.32±0.12) pg/ml、(41.15±11.72) pg/ml、(490.00±123.36) pg/ml.IL-13、TSLP在成年组、未成年组患者中升高,具有统计学意义(t=1.27,P＜0.05；t=3.11,P＜0.05).在有典型并殖吸虫病流行病学特征的未成年组中,IL-5升高(t=3.11,P＜0.01),具有统计学意义；而在无典型症状的成年组中的变化无统计学意义(t=1.52,P＞0.05).IL-4在成年组、未成年组患者中均无统计学意义变化(t=1.08,P＞0.05；t=2.1,P＞0.05).结论并殖吸虫病患者Th2免疫反应活化,IL-5、IL-13、TSLP显著上调,可以作为潜在的诊断标志物进行下一步的研究筛选.
목적 연구병식흡충감염자Th2면역응답산생적세포인자IL-5、IL-4、IL-13、흉선기질림파세포생성소(thymic stromal lymphopoietin,TSLP)적수평,사선출가이용우림상진단병식흡충병적잠재세포인자표지물.방법 수집귀주성본지병식흡충병환자성년조(년령＞18주세)급미성년조환자(년령≤18주세)적혈청양본각12례,이건강인혈청4례위대조,ELISA검측혈청중적IL-5、IL-4、IL-13、TSLP등4충세포인자적농도,각병례조급대조조간채용독립양본t검험분석,동시수집환자적류행병학신식.결과 성년조IL-5、IL-4、IL-13、TSLP분비농도분별위(19.50±5.57)pg/ml、(0.95±0.19)pg/ml、(475.30±629.81) pg/ml、(16 676.67±7 169.29) pg/ml；미성년조분별위(23.04±3.37)pg/ml、(1.05 ±0.19) pg/ml、(422.84±539.48) pg/ml、(16 242.50±6 230.81) pg/ml；대조조분별위(4.45±0.84) pg/ml、(0.32±0.12) pg/ml、(41.15±11.72) pg/ml、(490.00±123.36) pg/ml.IL-13、TSLP재성년조、미성년조환자중승고,구유통계학의의(t=1.27,P＜0.05；t=3.11,P＜0.05).재유전형병식흡충병류행병학특정적미성년조중,IL-5승고(t=3.11,P＜0.01),구유통계학의의；이재무전형증상적성년조중적변화무통계학의의(t=1.52,P＞0.05).IL-4재성년조、미성년조환자중균무통계학의의변화(t=1.08,P＞0.05；t=2.1,P＞0.05).결론 병식흡충병환자Th2면역반응활화,IL-5、IL-13、TSLP현저상조,가이작위잠재적진단표지물진행하일보적연구사선.
Objective To investigate the cytokines including IL-5,IL-4,IL-13 and thymic stromal lymphopoietin (TSLP) levels of Th2 immune response to Paragonimus infections,thus to find the potentialbiomarkers for clinical diagnosis on parasitic worms infections.Methods The sera of local paragonimiasis patients from adult ( ＞ 18 years old) and juvenile ( ≤ 18 years old) group in Guizhou Province were collected,respectively.The concentration of IL-5,IL-4,IL-13 and TSLP was measured by ELISA and analyzed with independent sample t test among each patient group and control group.Simultaneously the epidemical data of patients were investigated.Results The concentration of IL-5,IL-4,IL-13 and TSLP in adult group was (9.50 ±5.57) pg/ml,(0.95 ±0.19) pg/ml,(475.30 ±629.81) pg/ml and (16 676.67 ±7 169.29) pg/ml,respectively while in juvenile group was (23.04 ± 3.37) pg/ml,(1.05 ± 0.19) pg/ml,(422.84 ± 539.48 )pg/ml and (16 242.50 ± 6230.81 ) pg/ml,respectively.Meanwhile the concentration in control group was (4.45 ±0.84) pg/ml,(0.32 ± 0.12) pg/ml,(41.15 ± 11.72) pg/ml,(490.00 ± 123.36) pg/ml,respectively.IL-13 and TSLP increased significantly in two patient groups (t =1.27,P ＜ 0.05; t =3.11,P ＜0.05).IL-5 increased significantly in juvenile with typical paragonimiasis epidemiology features (t =3.11,P ＜0.01 ).On the contrary,IL-5 had no significant change in adults without typical symptoms (t =1.52,P ＞0.05).The variation of IL-4 in two patient groups had no statistical significance ( t =1.08,P ＞ 0.05 ; t =2.1,P ＞ 0.05).Conclusion Th2 immune response was activated in paragonimiasis patients with significant up regulation of IL-5,IL-13,and TSLP.The cytokines are of worth to be further researched and screened as potential biomarkers.