国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2012年
1期
1-5
,共5页
迟翔宇%肖伟%王学群%李青%王静%姜洪娟%王来城%王建平
遲翔宇%肖偉%王學群%李青%王靜%薑洪娟%王來城%王建平
지상우%초위%왕학군%리청%왕정%강홍연%왕래성%왕건평
ADAM33%基因多态性%相关性%哮喘
ADAM33%基因多態性%相關性%哮喘
ADAM33%기인다태성%상관성%효천
ADAM33%Single nucleotide Polymorphism%Association%Asthma
目的 检测ADAM33基因位点多态性在支气管哮喘(简称哮喘)患者中的分布频率,探讨ADAM33单核苷酸多态性与哮喘的相关性.方法 采用等位基因特异性聚合酶链反应(Allele-Specific Polymerase chain reaction,AS-PCR)技术及DNA测序的方法,对126例哮喘患者及121名健康人进行ADAM33基因F+1、S2、T2和V4位点单核苷酸多态性分析.结果 ADAM33基因位点病例组和对照组中基因型分布均符合Hardy- Weinburg平衡定律;哮喘组与对照组F+1、T2和V4位点基因型比较差异无统计学意义(x2 =1.638,P=0.441>0.05;x2=1.050,P=0.592>0.05;x2=0.310,P=0.856>0.05).哮喘组与对照组S2位点基因型及等位基因频率差异均具有统计学意义(x2=6.929,P<0.05).根据病情严重程度把哮喘患者分为轻、中、重3组,经非参数检验分析,S2位点x2值为0.335,P=0.855>0.05,S2位点多态性与哮喘病情严重度差异无统计学意义.方差分析计算基因型构成和肺功能指标FEV1实/预、FVC实/预和FEV1/FVC的关联,S2位点的P值分别为0.255、0.143、0.404,均>0.05,S2位点多态性与肺功能指标无显著相关性.结论 ADAM33基因位点在哮喘人群中存在多态性;ADAM33基因S2位点基因多态性与哮喘明显相关,与哮喘患者病情严重程度和肺功能没有显著关联.
目的 檢測ADAM33基因位點多態性在支氣管哮喘(簡稱哮喘)患者中的分佈頻率,探討ADAM33單覈苷痠多態性與哮喘的相關性.方法 採用等位基因特異性聚閤酶鏈反應(Allele-Specific Polymerase chain reaction,AS-PCR)技術及DNA測序的方法,對126例哮喘患者及121名健康人進行ADAM33基因F+1、S2、T2和V4位點單覈苷痠多態性分析.結果 ADAM33基因位點病例組和對照組中基因型分佈均符閤Hardy- Weinburg平衡定律;哮喘組與對照組F+1、T2和V4位點基因型比較差異無統計學意義(x2 =1.638,P=0.441>0.05;x2=1.050,P=0.592>0.05;x2=0.310,P=0.856>0.05).哮喘組與對照組S2位點基因型及等位基因頻率差異均具有統計學意義(x2=6.929,P<0.05).根據病情嚴重程度把哮喘患者分為輕、中、重3組,經非參數檢驗分析,S2位點x2值為0.335,P=0.855>0.05,S2位點多態性與哮喘病情嚴重度差異無統計學意義.方差分析計算基因型構成和肺功能指標FEV1實/預、FVC實/預和FEV1/FVC的關聯,S2位點的P值分彆為0.255、0.143、0.404,均>0.05,S2位點多態性與肺功能指標無顯著相關性.結論 ADAM33基因位點在哮喘人群中存在多態性;ADAM33基因S2位點基因多態性與哮喘明顯相關,與哮喘患者病情嚴重程度和肺功能沒有顯著關聯.
목적 검측ADAM33기인위점다태성재지기관효천(간칭효천)환자중적분포빈솔,탐토ADAM33단핵감산다태성여효천적상관성.방법 채용등위기인특이성취합매련반응(Allele-Specific Polymerase chain reaction,AS-PCR)기술급DNA측서적방법,대126례효천환자급121명건강인진행ADAM33기인F+1、S2、T2화V4위점단핵감산다태성분석.결과 ADAM33기인위점병례조화대조조중기인형분포균부합Hardy- Weinburg평형정률;효천조여대조조F+1、T2화V4위점기인형비교차이무통계학의의(x2 =1.638,P=0.441>0.05;x2=1.050,P=0.592>0.05;x2=0.310,P=0.856>0.05).효천조여대조조S2위점기인형급등위기인빈솔차이균구유통계학의의(x2=6.929,P<0.05).근거병정엄중정도파효천환자분위경、중、중3조,경비삼수검험분석,S2위점x2치위0.335,P=0.855>0.05,S2위점다태성여효천병정엄중도차이무통계학의의.방차분석계산기인형구성화폐공능지표FEV1실/예、FVC실/예화FEV1/FVC적관련,S2위점적P치분별위0.255、0.143、0.404,균>0.05,S2위점다태성여폐공능지표무현저상관성.결론 ADAM33기인위점재효천인군중존재다태성;ADAM33기인S2위점기인다태성여효천명현상관,여효천환자병정엄중정도화폐공능몰유현저관련.
Objective The aim of this study was to evaluate the potential relationship between polymorphisms of ADAM33 and bronchial asthma (asthma).Methods A case control study was conducted.A total of 126 asthma patients and a control group of 121 healthy volunteers were recruited for this study.Four polymorphic sites (F+1,S2,T2,and V4) were selected for genotyping.Genotypes were determined by the Allele Specific Polymerase chain reaction (AS-PCR) with Fluorescence Melting Curves and DNA sequencing method.Results Statistically significant difference in the distributions of the S2 site between patients and controls was observed ( x2 =6.929,P <0.05).No significant differences were found with asthma severity ( x2 =0.335,P =0.855>0.05) and lung function.Conclusions These preliminary results suggest an association between ADAM33 polymorphisms S2 C/G and asthma. The SNP (F+1 C/T,T2 G/A,and V4 C/G) of the ADAM33 gene may be the causal variants in asthma disease,but the strength of this evidence is limited by our small sample size.