中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2010年
10期
607-610
,共4页
吐尔洪江·吐逊%吐尔干·艾力%单骄宇%张雪%阿尔孜古丽·吐逊%刘弓伯%林仁勇%温浩
吐爾洪江·吐遜%吐爾榦·艾力%單驕宇%張雪%阿爾孜古麗·吐遜%劉弓伯%林仁勇%溫浩
토이홍강·토손%토이간·애력%단교우%장설%아이자고려·토손%류궁백%림인용%온호
棘球蚴病,肝%T淋巴细胞,辅助诱导%白细胞介素17%白细胞介素23%染色与标记
棘毬蚴病,肝%T淋巴細胞,輔助誘導%白細胞介素17%白細胞介素23%染色與標記
극구유병,간%T림파세포,보조유도%백세포개소17%백세포개소23%염색여표기
Echinococcosis,hepatic%T-lymphocytes,helper-inducer%Interleukin-17%Interlenkin-23%Staining and labeling
目的 探讨肝囊型包虫病患者外周血中辅助性T淋巴细胞(Th)17的变化.方法 将56例受试者分为:健康志愿者(HD)组20例,肝囊型包虫病(CE)组18例,肝囊型包虫病合并胆瘘(BF)组18例.流式细胞仪胞内染色分析法检测各组受试者外周血中Th17细胞占CD4+T淋巴细胞比例,ELlSA检测血清Th17细胞相关细胞因子IL-17、IL-23水平.数据行t检验和Pearson相关分析.结果 CE组患者外周血Th17/CD4+T淋巴细胞比例为(0.23±0.11)%,明显低于BF组的(0.76±0.43)%和HD组的(0.52±0.50)%(t=2.225,t=4.077,均P＜0.05),而BF组和HD组间差异无统计学意义(t=1.931,P＞0.05).血清IL-17和IL-23在CE组分别为(12.1±3.7)ng/L和(84.4±46.0)ng/L,明显低于BF组的(15.5±4.1)ng/L和(138.6±37.9)ng/L(t=2.515,t=3.649,均P＜0.05),也低于HD组的(14.8±4.4)ng/L和(138.1±48.7)ng/L(t=2.401,t=3.706,均P＜0.05),而BF组和HD组间差异无统计学意义(t=0.534,P＞0.05).各组血清IL-17水平与IL-23水平呈正相关(r=0.657,P＜0.05).结论 肝囊型包虫病患者外周血中Th17细胞比例明显减少,血清IL-17、IL-23水平显著降低.
目的 探討肝囊型包蟲病患者外週血中輔助性T淋巴細胞(Th)17的變化.方法 將56例受試者分為:健康誌願者(HD)組20例,肝囊型包蟲病(CE)組18例,肝囊型包蟲病閤併膽瘺(BF)組18例.流式細胞儀胞內染色分析法檢測各組受試者外週血中Th17細胞佔CD4+T淋巴細胞比例,ELlSA檢測血清Th17細胞相關細胞因子IL-17、IL-23水平.數據行t檢驗和Pearson相關分析.結果 CE組患者外週血Th17/CD4+T淋巴細胞比例為(0.23±0.11)%,明顯低于BF組的(0.76±0.43)%和HD組的(0.52±0.50)%(t=2.225,t=4.077,均P＜0.05),而BF組和HD組間差異無統計學意義(t=1.931,P＞0.05).血清IL-17和IL-23在CE組分彆為(12.1±3.7)ng/L和(84.4±46.0)ng/L,明顯低于BF組的(15.5±4.1)ng/L和(138.6±37.9)ng/L(t=2.515,t=3.649,均P＜0.05),也低于HD組的(14.8±4.4)ng/L和(138.1±48.7)ng/L(t=2.401,t=3.706,均P＜0.05),而BF組和HD組間差異無統計學意義(t=0.534,P＞0.05).各組血清IL-17水平與IL-23水平呈正相關(r=0.657,P＜0.05).結論 肝囊型包蟲病患者外週血中Th17細胞比例明顯減少,血清IL-17、IL-23水平顯著降低.
목적 탐토간낭형포충병환자외주혈중보조성T림파세포(Th)17적변화.방법 장56례수시자분위:건강지원자(HD)조20례,간낭형포충병(CE)조18례,간낭형포충병합병담루(BF)조18례.류식세포의포내염색분석법검측각조수시자외주혈중Th17세포점CD4+T림파세포비례,ELlSA검측혈청Th17세포상관세포인자IL-17、IL-23수평.수거행t검험화Pearson상관분석.결과 CE조환자외주혈Th17/CD4+T림파세포비례위(0.23±0.11)%,명현저우BF조적(0.76±0.43)%화HD조적(0.52±0.50)%(t=2.225,t=4.077,균P＜0.05),이BF조화HD조간차이무통계학의의(t=1.931,P＞0.05).혈청IL-17화IL-23재CE조분별위(12.1±3.7)ng/L화(84.4±46.0)ng/L,명현저우BF조적(15.5±4.1)ng/L화(138.6±37.9)ng/L(t=2.515,t=3.649,균P＜0.05),야저우HD조적(14.8±4.4)ng/L화(138.1±48.7)ng/L(t=2.401,t=3.706,균P＜0.05),이BF조화HD조간차이무통계학의의(t=0.534,P＞0.05).각조혈청IL-17수평여IL-23수평정정상관(r=0.657,P＜0.05).결론 간낭형포충병환자외주혈중Th17세포비례명현감소,혈청IL-17、IL-23수평현저강저.
Objective To investigate the change of T helper 17 cells (Th17) in the peripheral blood mononuclear cell (PBMC) in patients with liver cystic echinococcosis. Methods Fifty-six subjects were divided into three groups: healthy controls (HD, n = 20), patients with cystic echinococcosis (CE, n= 18) and patients with cystic echinococcosis combined with bile fistula (BF,n= 18). The frequency of Th17 cells in CD4+ T lymphocytes was detected by flow cytometry. Th17-related cytokines including interleukin (IL)-17 and IL-23 were measured by enzyme-linked immunosorbent assay (ELISA). The data were analyzed by t test and Pearson correlation analysis.Results The frequency of Th17 in the peripheral blood was significantly lower in CE group compared to BF group and HD group [(0. 23±0. 11)% vs (0. 76±0.43)% vs (0.52±0.50)%; t=2. 225 and4. 077 respectively, both P＜0.05), while there was no statistical difference between BF group and HD group (t=1. 931, P＞0.05). The levels IL-17 and IL-23 were (12.1±3.7) ng/L and (84.4±46.0) ng/L respectively in CE group, which were lower than those in BF group [(15.5±4.1) ng/L and (138.6±37. 9) ng/L, respectively; t=2. 515 and 3. 649 respectively, both P＜0.05] and those in HD group [(14.8±4.4) ng/L and (138.1±48. 7) ng/L, respectively; t=2. 401 and 3. 706 respectively,both P ＜0.05], while there was no statistical difference between BF group and HD group (t=0. 534,P ＞0.05). Serum concentrations of IL-17 were all positively correlated with the concentrations of IL23 in these three groups (r=0. 657, P＜0.05). Conclusion The frequeny of Th17 cells in PBMC and the serum concentrations of IL-17, IL-23 are significantly reduced in patients with cystic echinococcosis.
