CAJ | 학술논문

目的将从大鼠嗅黏膜中获取的鼻嗅黏膜固有层(OLP)组织薄片移植到大鼠脊髓横断区,同时应用神经节苷脂(GM1)治疗,观察其对损伤脊髓的修复作用.方法采用SD大鼠胸脊髓半切损伤模型,随机分为5组:OLP+GM1组、GM1组、OLP组,单纯损伤组、空白对照组.术后应用电生理学及病理学检查,观察大鼠神经功能恢复情况.结果 OLP+GM1组部分大鼠出现刺激右后肢的逃避反应,其余各组运动功能无明显改善.组织学检查显示,OLP+GM1组嗅组织在移植区成活,并沿着一定路线辐射,核转录因子阳性纤维穿行于移植区.与GM1、OLP,单纯损伤组比较,术后4周OLP+GM1组N1波潜伏期(4.71±0.72)ms(P<0.01);术后8周OLP+GM1组核转录因子(NF)密度(7.31±0.26)×104个/mm2(P<0.05).结论鼻嗅组织移植及GM1联合治疗脊髓损伤,具有明显的协同作用.
목적 장종대서후점막중획취적비후점막고유층(OLP)조직박편이식도대서척수횡단구,동시응용신경절감지(GM1)치료,관찰기대손상척수적수복작용.방법 채용SD대서흉척수반절손상모형,수궤분위5조:OLP+GM1조、GM1조、OLP조,단순손상조、공백대조조.술후응용전생이학급병이학검사,관찰대서신경공능회복정황.결과 OLP+GM1조부분대서출현자격우후지적도피반응,기여각조운동공능무명현개선.조직학검사현시,OLP+GM1조후조직재이식구성활,병연착일정로선복사,핵전록인자양성섬유천행우이식구.여GM1、OLP,단순손상조비교,술후4주OLP+GM1조N1파잠복기(4.71±0.72)ms(P<0.01);술후8주OLP+GM1조핵전록인자(NF)밀도(7.31±0.26)×104개/mm2(P<0.05).결론 비후조직이식급GM1연합치료척수손상,구유명현적협동작용.
Objective To observe the effects of olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment on spinal cord injury (SCI) in rats.Methods Totally 50 healthy pure breed female Sprague-Dawley (SD) rats after spinal cord hemiseetion were randomly divided into 5 groups and were given different treatments: (OLP + GM1) treatment group (group A), GM1 treatment group (group B), OLP treatment group (group C), spinal cord injury but without treatment group (group D) and healthy control group (group E). The recovery of neurological function was evaluated by somatosensory evoked potential (SEP) and pathological examination after surgery. Results In group A, in some rats an escaping response in right hind leg occurred, but in other groups, the motor function was not significantly improved. Histological examination showed that transplanted olfactory lamina propria survived in the transplantation area and expanded on certain routes. NF positive nerve fibers passed through the transplantation area. Compared with group B, C, D, the N1-wave latency was(4.71±0. 72)ms 4 weeks after operation(P<0. 01), and the NF density was(7. 31±0. 26) ×104/mm28 weeks after operation in group A(P<0. 05). Conclusions Olfactory lamina propria (OLP) transplantation and ganglioside GM1 treatment have a synergistic effect on SCI.