CAJ | 학술논문

目的观察恩度对人脐静脉内皮细胞(HUVECs)中MicroRNAs(miRNAs)表达的影响,探讨恩度通过调控MicroRNAs发挥抗血管生成的作用机制.方法采用实时荧光定量逆转录-聚合酶链反应(QRT-PCR)技术,检测50、100、200、500μg/L恩度对HUVECs干预前及干预1、2、4、8 h后miR126及miR221表达水平,并用2-△△CT法计算相对表达的倍数变化.结果 miR126、miR221在HUVECs中均高表达;恩度显著下凋miR126的表达(0.07±0.01～0.56±0.04,P＜0.01);相反,恩度明显上调miR221表达(1.45±0.06～8.87±1.51,P＜0.01);干预前后,miR126及miR221表达水平差异有统计学意义(P＜0.05).结论恩度显著影响HUVECs中miR126、miR221表达,提示恩度存在通过调控MicroRNAs发挥抗血管生成作用的机制.
목적 관찰은도대인제정맥내피세포(HUVECs)중MicroRNAs(miRNAs)표체적영향,탐토은도통과조공MicroRNAs발휘항혈관생성적작용궤제.방법 채용실시형광정량역전록-취합매련반응(QRT-PCR)기술,검측50、100、200、500μg/L은도대HUVECs간예전급간예1、2、4、8 h후miR126급miR221표체수평,병용2-△△CT법계산상대표체적배수변화.결과 miR126、miR221재HUVECs중균고표체;은도현저하조miR126적표체(0.07±0.01～0.56±0.04,P＜0.01);상반,은도명현상조miR221표체(1.45±0.06～8.87±1.51,P＜0.01);간예전후,miR126급miR221표체수평차이유통계학의의(P＜0.05).결론 은도현저영향HUVECs중miR126、miR221표체,제시은도존재통과조공MicroRNAs발휘항혈관생성작용적궤제.
Objective To investigate the effects of Endostar on the expression of microRNAs in the human umbilical vascular endothelial cells (HUVECs) and the anti - angiogenesis mechanism of Endostar by modulating microRNAs. Methods The expression levels of miR126 and miR221, which were interfered with different concentrations of Endostar (50, 100,200,500 μg/L) for different durations (1,2, 4, 8 h), were detected by real-time fluorescent quantitation reverse transcription-polymerase chain reaction (QRT-PCR). Relative gene expression data were analyzed by 2 - △△Cr method. Results Over expression of miR126 and miR221 was detected in the HUVECs. The expression of miR126 was down regulated by Endostar significantly (0.07 ±0.01- 0.56 ±0.04,P ＜0.01 ), as compared with that before interference by Endostar. The expression of miR221 was obviously up - regulated by Endostar ( 1.45 ± 0.06 -8.87 ±1.51 ,P ＜ 0.01 ), as compared with that before interference by Endostar. Conclusion Endostar can regulate the expression of miR126 and miR221 in the HUVECs, suggesting that Endostar exerts its anti - angiogenesis by modulating microRNAs.