CAJ | 학술논문

目的探讨α-平滑肌肌动蛋白(α-SMA)在肝癌中的表达及与肝癌侵袭转移的关系.方法收集非肝癌的肝脏组织15例,肝癌并伴门静脉转移的癌组织30例,按病理分化程度分为3组:低分化组、中分化组和高分化组.采取逆转录-聚合酶链反应(RT-PCR)检测肝癌组织中α-SMAmRNA的表达和免疫组织化学的方法检测α-SMA在癌组织及门静脉癌栓组织的表达.结果 RT-PCR结果显示α-SMA在所有肝癌组织中均呈阳性表达；免疫组织化学显示α-SMA在对照肝脏、肝癌组织和门脉癌栓组织的表达值分别为125.69±29.17、187.43±17.66、218.70±13.52,方差分析显示α-SMA表达差异有统计学意义(P＜0.01),门脉癌栓组表达最高；按照肝脏病理分为3组:低分化组、中分化组和高分化组,表达值分别为237.14±25.83、190.57±19.90、165.67±22.64,方差分析显示α-SMA表达差异有统计学意义(P＜0.01),在低分化组表达最高.结论 α-SMA在肝癌中表达明显增加,特别是门静脉癌栓组及低分化组,与肝癌的侵袭、转移有关.
목적 탐토α-평활기기동단백(α-SMA)재간암중적표체급여간암침습전이적관계.방법 수집비간암적간장조직15례,간암병반문정맥전이적암조직30례,안병리분화정도분위3조:저분화조、중분화조화고분화조.채취역전록-취합매련반응(RT-PCR)검측간암조직중α-SMAmRNA적표체화면역조직화학적방법검측α-SMA재암조직급문정맥암전조직적표체.결과 RT-PCR결과현시α-SMA재소유간암조직중균정양성표체；면역조직화학현시α-SMA재대조간장、간암조직화문맥암전조직적표체치분별위125.69±29.17、187.43±17.66、218.70±13.52,방차분석현시α-SMA표체차이유통계학의의(P＜0.01),문맥암전조표체최고；안조간장병리분위3조:저분화조、중분화조화고분화조,표체치분별위237.14±25.83、190.57±19.90、165.67±22.64,방차분석현시α-SMA표체차이유통계학의의(P＜0.01),재저분화조표체최고.결론 α-SMA재간암중표체명현증가,특별시문정맥암전조급저분화조,여간암적침습、전이유관.
Objective To study on the expression of α-smooth muscle actin (α-SMA) in liver cancer and its relation with the invasion and metastasis of liver cancer.Methods Collect liver tissues including no liver cancer tissues,liver cancer and with metastasis in portal vein; divide them into three groups in accordance with the pathologic differentiation degree:low differentiation group,medium differentiation group and high differentiation group.Use reverse transcription-polymerase chain reaction (RT-PCR)to test the expression of α-SMA mRNA in the tissues of liver cancer,and use the method of immunohistochemistry to test expression of α-SMA in the cancer tissue and the tumor thrombus tissues in portal vein.Results The result of RT-PCR shows that α-SMA expression is positive in all the tissues of liver cancer; the result of immunohistochemistry shows that the expression values of the control liver tissues,tissues with liver cancer and tumor thrombus tissues in portal vein are 125.69 ±29.17,187.43 ± 17.66 and 218.70 ±13.52 respectively,Variance analysis shows that the difference of α-SMA expression has statistically significance (P ＜0.01 ),of which,tumor thrombus tissues in portal vein have the highest expression value; the expression values of low differentiation group,medium differentiation group and high differentiation group are 237.14 ±25.83,190.57 ± 19.90 and 165.67 ±22.64 respectively.The variance analysis shows that the difference of α-SMA expression has statistically significance ( P ＜ 0.01 ),which is the highest in the low differentiation group.Conclusion There is a significant increase expression of α-SMA in liver cancer,especially in the group of tumor thrombus tissues in portal vein and the low differentiation group.It is related to the invasion and metastasis of liver cancer.