肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2011年
1期
14-17
,共4页
王鹏%饶晓松%李勇%宁涛%刘宝国
王鵬%饒曉鬆%李勇%寧濤%劉寶國
왕붕%요효송%리용%저도%류보국
人类乳头瘤病毒%喉肿瘤%聚合酶链反应%原位杂交
人類乳頭瘤病毒%喉腫瘤%聚閤酶鏈反應%原位雜交
인류유두류병독%후종류%취합매련반응%원위잡교
Human papillomavirus%Laryngeal neoplasms%Polymerase chain reaction%In situ hybridization
目的 探讨人类乳头瘤病毒(HPV)感染与喉鳞状细胞癌(LSCC)的关系.方法 采用多对引物进行组织DNA聚合酶链反应(PCR)及原位杂交(ISH)技术对84例LSCC组织进行检测,从多角度、多方面印证HPV的感染.结果 PCR检出HPV-L1阳性率为27.4%(23/84),而针对HPV-16型、HPV-18型特异的E6/E7引物行PCR扩增的结果显示,29例(34.5%)为HPV-16型,6例(7.1%)为HPV-18型,其中4例(4.8%)为HPV-16、HPV-18混合感染.LSCC中HPV-16和HPV-18的总阳性率为36.9%.用地高辛标记的HPV-16E6探针进行的ISH结果显示喉癌组织中HPV-16E6 mRNA的检出率为30.9%(26/84).结论 高危型HPV-16的感染可能参与LSCC的致癌过程,但其具体机制尚待进一步研究.
目的 探討人類乳頭瘤病毒(HPV)感染與喉鱗狀細胞癌(LSCC)的關繫.方法 採用多對引物進行組織DNA聚閤酶鏈反應(PCR)及原位雜交(ISH)技術對84例LSCC組織進行檢測,從多角度、多方麵印證HPV的感染.結果 PCR檢齣HPV-L1暘性率為27.4%(23/84),而針對HPV-16型、HPV-18型特異的E6/E7引物行PCR擴增的結果顯示,29例(34.5%)為HPV-16型,6例(7.1%)為HPV-18型,其中4例(4.8%)為HPV-16、HPV-18混閤感染.LSCC中HPV-16和HPV-18的總暘性率為36.9%.用地高辛標記的HPV-16E6探針進行的ISH結果顯示喉癌組織中HPV-16E6 mRNA的檢齣率為30.9%(26/84).結論 高危型HPV-16的感染可能參與LSCC的緻癌過程,但其具體機製尚待進一步研究.
목적 탐토인류유두류병독(HPV)감염여후린상세포암(LSCC)적관계.방법 채용다대인물진행조직DNA취합매련반응(PCR)급원위잡교(ISH)기술대84례LSCC조직진행검측,종다각도、다방면인증HPV적감염.결과 PCR검출HPV-L1양성솔위27.4%(23/84),이침대HPV-16형、HPV-18형특이적E6/E7인물행PCR확증적결과현시,29례(34.5%)위HPV-16형,6례(7.1%)위HPV-18형,기중4례(4.8%)위HPV-16、HPV-18혼합감염.LSCC중HPV-16화HPV-18적총양성솔위36.9%.용지고신표기적HPV-16E6탐침진행적ISH결과현시후암조직중HPV-16E6 mRNA적검출솔위30.9%(26/84).결론 고위형HPV-16적감염가능삼여LSCC적치암과정,단기구체궤제상대진일보연구.
Objective To investigate the relationship between human papilloma virus(HPV)infection and the development of laryngeal squamous cell carcinoma(LSCC). Methods To elucidate the role of HPV in the development of LSCC,we employed polymerase chain reaction(PCR)based on four pairs of primers an4 in situ hybridization(ISH)to screen the HPV infection in 84 ISCC tissues.Results Using HPV L1 general primer amplification,HPV DNA was detected in 23(27.4%)of the 84 LSCC samples.However,when specific primers for HPV-16 or-18 were used to amplify E6 and E7 in all samples,29 cases(34.5%)were positive for HPV-16,while 6 cases(7.1%)were positive for HPV 18.Coinfeetion of HPV-16 and-18 were found in 4cases (4.8%).Overall,HPV type 16 and 18 infections were present in 36.9% of the LSCC samples.In addition,the positive rate of HPV 16 E6 mRNA was 30.9%(26/84)in LSCC by ISH with digoxin-labeled sense probes of HPV 16 E6.Conclusion High-risk HPV-16may be an etiologic factor in the development of laryngeal squamous cell carcinoma, while the complicated molecular mechanism of HPV16 inducedtumorgenesis needs a further study.
