中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2009年
1期
98-101
,共4页
穆会君%谢平%沈云峰%蒋元强
穆會君%謝平%瀋雲峰%蔣元彊
목회군%사평%침운봉%장원강
钙粘蛋白13%BCR/ABL融合基因%慢性粒细胞白血病
鈣粘蛋白13%BCR/ABL融閤基因%慢性粒細胞白血病
개점단백13%BCR/ABL융합기인%만성립세포백혈병
cadherin-13%BCR/ABL fusion gene%chronic myeloid leukemia
目的 观察钙粘蛋白13(cadherin-13,CDH13)与BCR/ABL融合基因在不同临床阶段慢性粒细胞白血病(chronic myeloid leukemia,CML)患者中的表达变化及其相关性.方法 利用EVA Green建立实时荧光逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测CDH13、BCR/ABL和磷酸甘油醛脱氢酶(glyceraldehyde phosphate dehydrogenase,GAPDH)的方法 ,检测25例CML初发和25例CML急变患者的外周血样本中CDH13基因和BCR/ABL融合基因的表达,以及30名正常人外周血样本中CDH13基因的表达,以GAPDH为内参基因,各组基冈表达水平的差异采用△△CT相对定量法.结果 CML急变患者外周血BCR/ABL融合基因的表达水平是初发患者的4.72倍,CML初发和急变患者较正常人CDH13的表达水平降低,分别是正常人的36%和25%,CDH13的表达水平与BCR/ABL融合基因的表达负相关.结论 CDH13在CML不同临床阶段都表达降低,这可能是CML粘附缺陷的原因之一,CDH13表达可能受到BCR/ABL融合基因的调控.
目的 觀察鈣粘蛋白13(cadherin-13,CDH13)與BCR/ABL融閤基因在不同臨床階段慢性粒細胞白血病(chronic myeloid leukemia,CML)患者中的錶達變化及其相關性.方法 利用EVA Green建立實時熒光逆轉錄聚閤酶鏈反應(reverse transcription polymerase chain reaction,RT-PCR)檢測CDH13、BCR/ABL和燐痠甘油醛脫氫酶(glyceraldehyde phosphate dehydrogenase,GAPDH)的方法 ,檢測25例CML初髮和25例CML急變患者的外週血樣本中CDH13基因和BCR/ABL融閤基因的錶達,以及30名正常人外週血樣本中CDH13基因的錶達,以GAPDH為內參基因,各組基岡錶達水平的差異採用△△CT相對定量法.結果 CML急變患者外週血BCR/ABL融閤基因的錶達水平是初髮患者的4.72倍,CML初髮和急變患者較正常人CDH13的錶達水平降低,分彆是正常人的36%和25%,CDH13的錶達水平與BCR/ABL融閤基因的錶達負相關.結論 CDH13在CML不同臨床階段都錶達降低,這可能是CML粘附缺陷的原因之一,CDH13錶達可能受到BCR/ABL融閤基因的調控.
목적 관찰개점단백13(cadherin-13,CDH13)여BCR/ABL융합기인재불동림상계단만성립세포백혈병(chronic myeloid leukemia,CML)환자중적표체변화급기상관성.방법 이용EVA Green건립실시형광역전록취합매련반응(reverse transcription polymerase chain reaction,RT-PCR)검측CDH13、BCR/ABL화린산감유철탈경매(glyceraldehyde phosphate dehydrogenase,GAPDH)적방법 ,검측25례CML초발화25례CML급변환자적외주혈양본중CDH13기인화BCR/ABL융합기인적표체,이급30명정상인외주혈양본중CDH13기인적표체,이GAPDH위내삼기인,각조기강표체수평적차이채용△△CT상대정량법.결과 CML급변환자외주혈BCR/ABL융합기인적표체수평시초발환자적4.72배,CML초발화급변환자교정상인CDH13적표체수평강저,분별시정상인적36%화25%,CDH13적표체수평여BCR/ABL융합기인적표체부상관.결론 CDH13재CML불동림상계단도표체강저,저가능시CML점부결함적원인지일,CDH13표체가능수도BCR/ABL융합기인적조공.
Objective To investigate the expression of the CDH13 gene and BCR/ABL fusion gene in chronic myeloid leukemia(CML)patients at different stages and explore their relationship.Methods RNA was isolated from peripheral blood in 30 healthy adults.22 primary CML patients and 25 blastic crisis of CML patients.We examined the expression of the CDH13 gene and BCR/ABL fusion gene using EVA Green real-time reverse transcriptase-polymerase chain reaction(RT-PCR).Results The expression of the BCR/ABL fusion gene in the patients with blastic crisis CML was 4.72 folds as that of the patients with primary CML.The expression of the CDH13 gene in the primary and blastic crisis CML patients was significantly reduced to 36%and 25%of that in healthy controls,respectively.Moreover,negative correlation was found between the expressions of these two genes.Conclusion The study showed that the reduction of the CDH13 expression at different clinical stage of CML may account for the defective cell adhesion in CML.and the expression of the CDH13 gene was probably down-regulated by the BCR/ABL fusion gene.
