中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2010年
12期
1108-1112
,共5页
金成豪%米满吉和%丁伟荣%居石克夫
金成豪%米滿吉和%丁偉榮%居石剋伕
금성호%미만길화%정위영%거석극부
血管内皮生长因子受体2%干细胞%细胞分化%细胞移植
血管內皮生長因子受體2%榦細胞%細胞分化%細胞移植
혈관내피생장인자수체2%간세포%세포분화%세포이식
Vascular endothelial growth factor receptor-2%Stem cell%Cell differentiation%Stem cell transplantation
目的 探讨胎肝中胎肝激酶-1(fetla liver kinase 1,FLK-1)阳性细胞的特征和功能.方法 免疫磁珠分离胎肝FLK-1阳性细胞,流式细胞术(又称荧光活化细胞分类计数,fluorescence activated cell sorter,FACS)检测胎肝FLK-1阳性细胞.体外生长因子诱导培养、测定FLK-1阳性细胞的分化、增殖活性.FLK-1阳性细胞植入75%肝切除的小鼠肝脏模型中,借助荧光解剖显微镜及免疫组织化学方法观察血管形成及细胞分化的情况.结果 FLK-1阳性细胞经生长因子诱导后,可表达典型的血管内皮细胞、平滑肌细胞的标志.75%肝切除的小鼠肝脏模型中,植入的FLK-1阳性细胞在肝脏的纤维囊中分化成血管细胞并形成血管.结论 胎鼠肝脏FLK-1阳性细胞具有血管祖细胞的功能,具有潜在的临床应用价值.
目的 探討胎肝中胎肝激酶-1(fetla liver kinase 1,FLK-1)暘性細胞的特徵和功能.方法 免疫磁珠分離胎肝FLK-1暘性細胞,流式細胞術(又稱熒光活化細胞分類計數,fluorescence activated cell sorter,FACS)檢測胎肝FLK-1暘性細胞.體外生長因子誘導培養、測定FLK-1暘性細胞的分化、增殖活性.FLK-1暘性細胞植入75%肝切除的小鼠肝髒模型中,藉助熒光解剖顯微鏡及免疫組織化學方法觀察血管形成及細胞分化的情況.結果 FLK-1暘性細胞經生長因子誘導後,可錶達典型的血管內皮細胞、平滑肌細胞的標誌.75%肝切除的小鼠肝髒模型中,植入的FLK-1暘性細胞在肝髒的纖維囊中分化成血管細胞併形成血管.結論 胎鼠肝髒FLK-1暘性細胞具有血管祖細胞的功能,具有潛在的臨床應用價值.
목적 탐토태간중태간격매-1(fetla liver kinase 1,FLK-1)양성세포적특정화공능.방법 면역자주분리태간FLK-1양성세포,류식세포술(우칭형광활화세포분류계수,fluorescence activated cell sorter,FACS)검측태간FLK-1양성세포.체외생장인자유도배양、측정FLK-1양성세포적분화、증식활성.FLK-1양성세포식입75%간절제적소서간장모형중,차조형광해부현미경급면역조직화학방법관찰혈관형성급세포분화적정황.결과 FLK-1양성세포경생장인자유도후,가표체전형적혈관내피세포、평활기세포적표지.75%간절제적소서간장모형중,식입적FLK-1양성세포재간장적섬유낭중분화성혈관세포병형성혈관.결론 태서간장FLK-1양성세포구유혈관조세포적공능,구유잠재적림상응용개치.
Objective To characterize the differential and proliferative activities of FLK-1+ cells derived from mouse fetal liver. Method The FLK-1+ fraction were enriched from the fetal liver using immunomagnetic method and their differential and proliferative activities were examined in culture medium and in vivo via transplantation of FLK-1 + cells into the inferior pole of the spleen of nine-week-old male C57BL/6 mice after two-thirds hepatectomy. Results In response to growth factors, FLK-1 + cells expressed typical lineage-specific markers for vascular endothelial cells, smooth muscle cells. Intrahepatic implantation of FLK-1 + cells resulted in the formation of blood vessels in the fibrous capsule of partially hepatectomized liver. Conclusion These results indicate that FLK-1 + cells derived from mouse fetal liver could differentiate into endothelial cells and smooth muscle cells and they may serve as potential stem cells for clinical cellbased therapy.
