CAJ | 학술논문

目的研究谷胱甘肽硫转移酶-π（CST-π）在人食管鳞癌中的表达及其与食管鳞癌临床病理的相互关系。方法应用免疫组织化学方法和sandwich酶联免疫吸附测定法（enzyme linked immunosorbent assay, ELISA）对143例食鳞癌石蜡标本和43例食管鳞癌患者的术前及术后血清标本进行检测。结果 GST-π在人食管鳞癌石蜡标本和43例食管鳞癌患者的术前及术后血清标本异有极显著性（P〈0.01）。GST-π在高分化食管鳞癌中的表达显著高于其在低分公食管鳞癌中的表达（P〈0.05）.GST-π在高分公食管鳞癌中的表达显著高天其在低分公食管鳞癌中的表达(P<0.05).食管鳞癌患者术前和术后血清CST-π含量平均值均显著高天正常人CST-π含量平均值,但两者差异无显著性(P<0.05).结论 GST-π含量平均值低于术前血清GST-π含量平均值,但两者差异著性(P<0.05).结论 GST-π是食管鳞癌的一个有应用价值的标志物,其与食管鳞癌的发生有关,可能是食管上皮癌变前的一个早期标志物.
목적연구곡광감태류전이매-π（CST-π）재인식관린암중적표체급기여식관린암림상병리적상호관계。방법응용면역조직화학방법화sandwich매련면역흡부측정법（enzyme linked immunosorbent assay, ELISA）대143례식린암석사표본화43례식관린암환자적술전급술후혈청표본진행검측。결과 GST-π재인식관린암석사표본화43례식관린암환자적술전급술후혈청표본이유겁현저성（P〈0.01）。GST-π재고분화식관린암중적표체현저고우기재저분공식관린암중적표체（P〈0.05）.GST-π재고분공식관린암중적표체현저고천기재저분공식관린암중적표체(P<0.05).식관린암환자술전화술후혈청CST-π함량평균치균현저고천정상인CST-π함량평균치,단량자차이무현저성(P<0.05).결론 GST-π함량평균치저우술전혈청GST-π함량평균치,단량자차이저성(P<0.05).결론 GST-π시식관린암적일개유응용개치적표지물,기여식관린암적발생유관,가능시식관상피암변전적일개조기표지물.
Objective To evaluate the expression of glutathione S-transferase-π(GST-π)in human esophageal squamous cell carcinoma (ESGG)and ith correlation with the clinicopathological data,Methods Immuuohistochemical method(Labeled avidin biotin metod)and ELISAwere used to detect the expression of glu-tathione S-transferase-πin 143 cases of formalin fixed,paraffin embedded ESCC tissue sections. Serum GST-πwas determined before and after operation in 43 patients with ESCC. Resuits GST-πwas positively stained immunohistochemically in 58.7%(84/143)of ESGG and 79.6%(109/139)of surrounding noncancerous esophageal tissues(P<0.05).The expression level of GST-π was significantly higher in well differentiated than poorly differentiated carcinomas(P<0.05).No significant correlation was observed between CST-πLevels and lymph node metastasis,pathological staging or long term survival. The preoperative and postoperative mean serum GST-πlevel in postoperative patients was lower than that in preoperative patients ,but the differ-ence was not significant. Conclusion GST-πis a useful tumor marker for human ESCC.