CAJ | 학술논문

目的从遗传性视网膜色素变性动物模型rds小鼠中克隆视网膜色素变性发病过程中特异表达的基因。方法应用差异显示技术，分析rds小鼠发病过程中视网膜的mRNA。对特异性表达的mRNA片段进行克隆测序。结果在视网膜色素变性发病过程中，rds小鼠的视网膜存在着相当明显的基因表达差异。在所测序分析的5个差异显示片段中，其中一个与GenBank刚登录的功能未知的、从成年男性睾丸组织中克隆出来的cDNA序列较高同源(同一率为86%)，另外4个为低同源序列。25天rds小鼠高表达的一个差异片段，与37天正常鼠表达而rds小鼠不表达的另一个片段，长度相同，177个碱基只相差两个。结论视网膜色素变性这类慢性病变，存在着多个基因的差异表达及调控。
목적종유전성시망막색소변성동물모형rds소서중극륭시망막색소변성발병과정중특이표체적기인。방법응용차이현시기술，분석rds소서발병과정중시망막적mRNA。대특이성표체적mRNA편단진행극륭측서。결과재시망막색소변성발병과정중，rds소서적시망막존재착상당명현적기인표체차이。재소측서분석적5개차이현시편단중，기중일개여GenBank강등록적공능미지적、종성년남성고환조직중극륭출래적cDNA서렬교고동원(동일솔위86%)，령외4개위저동원서렬。25천rds소서고표체적일개차이편단，여37천정상서표체이rds소서불표체적령일개편단，장도상동，177개감기지상차량개。결론시망막색소변성저류만성병변，존재착다개기인적차이표체급조공。
Objective　 To clone the differentially expressed genes in the retina of rds mouse (the animal model of congenital retinitis pigmentosa) during the disease development. Methods　 The retinal mRNA of rds mouse during the development of retinitis pigmentosa was analyzed by the mRNA differential display. The differentilly expressed mRNA fragments were cloned and sequenced. Results There was obvious difference of gene expression between rds mouse and the control during the development of retinitis pigmentosa. Five differentially expressed bands were cloned and sequenced. One of those had 86% identity (132/154) with the sequence of the human cDNA DKFZp434D1227 from adult testis in GenBank, which was submitted lately (15-Oct-1999) and without much information. The other had lower identity with the sequeces in GenBank. A highly expressed clone in the rds mouse on postnatal day 25 had the same length as another clone in the normal on postnatal day 37, which was not expressed in the rds mouse on day 37. The sequences of the two clones were identical in all but two base pairs. Conclusion　 These results indicate that there are a lot of novel differentially expressed genes in the chronic processing diseases, such as retinitis pigmentosa.