CAJ | 학술논문

目的研究我国A组轮状病毒感染状况及VP7血清型分布。方法利用PAGE电泳检测北京、沈阳、新乡、上海、深圳、广州六个城市1997和(或)1998年秋冬季婴幼儿腹泻A组轮状病毒的感染状况，并采用RT-PCR方法对4种主要的VP7血清型(G1、G2、G3和C4型)进行分型。结果 374份标本中有181份为轮状病毒阳性，检出率48.4％。各地标本的检出率在32％～63％之间，北京、上海地区两年的检出率基本近似。阳性标本经PCR分型方法鉴定G1、G2、G3和G4型的感染率分别为89.5％、6.1％、2.8％和0。有两例为G1和G2型混合感染，另有1例经测序证实为G9型。各地之间、每年之间RV的流行状况略有差异。结论 G1、G2和G3型是我国A组轮状病毒感染的主要血清型。
목적연구아국A조륜상병독감염상황급VP7혈청형분포。방법이용PAGE전영검측북경、침양、신향、상해、심수、엄주륙개성시1997화(혹)1998년추동계영유인복사A조륜상병독적감염상황，병채용RT-PCR방법대4충주요적VP7혈청형(G1、G2、G3화C4형)진행분형。결과 374빈표본중유181빈위륜상병독양성，검출솔48.4％。각지표본적검출솔재32％～63％지간，북경、상해지구량년적검출솔기본근사。양성표본경PCR분형방법감정G1、G2、G3화G4형적감염솔분별위89.5％、6.1％、2.8％화0。유량례위G1화G2형혼합감염，령유1례경측서증실위G9형。각지지간、매년지간RV적류행상황략유차이。결론 G1、G2화G3형시아국A조륜상병독감염적주요혈청형。
Objective To sutdy the prevalence of Group A rotavirus infection in China. Methods 374 samples from infants with diarrhea were collected from six cities (Beijing, Shenyang, Xinxiang, Shanghai, Shenzhen and Guangzhou)in autumn and winters of 1997 and/or 1998. The incidence of rotavirus infection and the prevalence of the four dominant serotypes G1, G2, G3, G4(VP7 serotype)were detected by PAGE and RT-PCR. Results Of the 374 samples detected, 181 were rotavirus positive. The positive rate was 48.4 %, ranging from 32%～63% in different cities. The positive rates between 1997 and 1998 in Beijing and Shanghai were similar. Serotyped by RT-PCR method based on VP7 sequence, the positive rates of type G1, G2, G3 and G4 was 89.5 %, 6.1%, 2.8 % and 0, respectively. Two cases were found being coinfected with G1 and G2, and one case was confirmed to be infected with G9 by cDNA sequence analysis. There was a little difference in prevalence of G type rotavirus infection among samples from different cities and different years. Conclusion The results indicated that rotavirus was the most important etiologic agent in this country, and the predominant serotypes were G1, G2 and G3.