CAJ | 학술논문

目的探讨IL-18在溃疡性结肠炎(UC)发病、发展和治疗中的作用.方法采用双抗体夹心ELISA法检测58例UC患者外周血中IL-18水平,并分别比较其与临床活动指数(CAI)和ESR、CRP等的相关性.结果 UC活动期患者血清IL-18水平较缓解期患者和健康对照者明显升高(P<0.05),而缓解期患者与健康对照者比较差异无统计学意义.重度UC患者血清IL-18水平[(392.78±50.17)pg/ml]明显高于中度UC患者[(138.92±23.41)pg/ml]和健康对照者[(73.76±20.27)pg/ml],但轻、中度UC之间差异无统计学意义.病变的范围越大,水平越高(P<0.05).并与临床指标ESR、CRP、WBC计数呈正相关.与CAI呈正相关(r=0.775,P<0.01).激素治疗后IL-18显著性下降.结论 IL-18可能参与UC的发病,血清IL-18可作为UC判断病情和活动性的参考指标.
목적 탐토IL-18재궤양성결장염(UC)발병、발전화치료중적작용.방법 채용쌍항체협심ELISA법검측58례UC환자외주혈중IL-18수평,병분별비교기여림상활동지수(CAI)화ESR、CRP등적상관성.결과 UC활동기환자혈청IL-18수평교완해기환자화건강대조자명현승고(P<0.05),이완해기환자여건강대조자비교차이무통계학의의.중도UC환자혈청IL-18수평[(392.78±50.17)pg/ml]명현고우중도UC환자[(138.92±23.41)pg/ml]화건강대조자[(73.76±20.27)pg/ml],단경、중도UC지간차이무통계학의의.병변적범위월대,수평월고(P<0.05).병여림상지표ESR、CRP、WBC계수정정상관.여CAI정정상관(r=0.775,P<0.01).격소치료후IL-18현저성하강.결론 IL-18가능삼여UC적발병,혈청IL-18가작위UC판단병정화활동성적삼고지표.
Objective To study the role of IL-18 in the pathogenesis and treatment of ulcerative colitis (UC). Method An enzyme-linked immunosorbent assay (ELISA) was utilized to detect the serum IL-18 level of 58 UC patients. Results The serum IL-18 level in acute period of UC patients was significantly higher than that in control ones(P < 0.05 ). There was no significant difference between remisson period of UC and control ones (P> 0.05). The serum IL-18 level was closely related with the degree of UC (P < 0.05),the mean concentration of serum IL-18 was significantly higher in patients with severe ulcerative colitis [ (392.78 ± 50.17)pg/ml]than in patients with mild colitis ulcerative [ (138.92 ± 23.41 )pg/ml]and in control ones. Serum IL-18 in active ulcerative colitis were positively related to clinical disease severity and activity or laboratory parameters,including CAI,serum CRP,erythrocyte sedimentation rates,or total leukocyte counts (r = 0.775,0.705,0.662,0.625,P < 0.01 ). The level of IL-18 was declined after treatment with corticoids(P< 0.05). Conclusions IL-18 might play an important role in the pathogenesis of UC. The measurement of IL-18 is helpful to estimate the disease activity of UC and it may be considered as laboratory and activity criteria for UC.