国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2010年
2期
119-122
,共4页
术后持续性疼痛%动物模型%疼痛行为学
術後持續性疼痛%動物模型%疼痛行為學
술후지속성동통%동물모형%동통행위학
Postoperative pain%Animal model%Pain behavior
目的 建立大鼠皮肤肌肉切口牵拉(skin/muscleincision and retraction,SMIR)术后持续性疼痛模型,研究SMIR术对术后疼痛的影响.方法 雄性SD大鼠36只,采用随机数字表法将大鼠随机分为空白对照组、假手术组和模型组,每组12只.按Flatters法制作SMIR持续性疼痛模型,应用von Frey纤维细丝法和Hargreaves法测定术前及术后第1、3、7、12、22、32天的机械缩足反射阈值(mechanical withdrawal threshold,MWT)和热缩足潜伏期(thermalwithdrawallatency,TWL);并且每组采用随机数字表法随机取4只于术后12d时电镜下观察手术侧隐神经损伤情况.结果 与空白对照组,模型组大鼠MWT在术后第1天即出现降低(P=0.015＜0.05);12 d时下降最为显著(P=0.001＜0.01),32d时差异无统计学意义(P=0.772＞0.05);而假手术组与空白对照组比较在各时间点MWT差异无统计学意义;整个观察时间过程中模型组大鼠TWL均无明显改变;SMIR术及假手术后隐神经均未出现髓鞘肿胀或脱髓鞘等神经损伤现象.结论 进一步证实了SMIR模型的稳定性和可重复性,将为研究术后持续性疼痛的机制及治疗措施提供一个新的平台.
目的 建立大鼠皮膚肌肉切口牽拉(skin/muscleincision and retraction,SMIR)術後持續性疼痛模型,研究SMIR術對術後疼痛的影響.方法 雄性SD大鼠36隻,採用隨機數字錶法將大鼠隨機分為空白對照組、假手術組和模型組,每組12隻.按Flatters法製作SMIR持續性疼痛模型,應用von Frey纖維細絲法和Hargreaves法測定術前及術後第1、3、7、12、22、32天的機械縮足反射閾值(mechanical withdrawal threshold,MWT)和熱縮足潛伏期(thermalwithdrawallatency,TWL);併且每組採用隨機數字錶法隨機取4隻于術後12d時電鏡下觀察手術側隱神經損傷情況.結果 與空白對照組,模型組大鼠MWT在術後第1天即齣現降低(P=0.015＜0.05);12 d時下降最為顯著(P=0.001＜0.01),32d時差異無統計學意義(P=0.772＞0.05);而假手術組與空白對照組比較在各時間點MWT差異無統計學意義;整箇觀察時間過程中模型組大鼠TWL均無明顯改變;SMIR術及假手術後隱神經均未齣現髓鞘腫脹或脫髓鞘等神經損傷現象.結論 進一步證實瞭SMIR模型的穩定性和可重複性,將為研究術後持續性疼痛的機製及治療措施提供一箇新的平檯.
목적 건립대서피부기육절구견랍(skin/muscleincision and retraction,SMIR)술후지속성동통모형,연구SMIR술대술후동통적영향.방법 웅성SD대서36지,채용수궤수자표법장대서수궤분위공백대조조、가수술조화모형조,매조12지.안Flatters법제작SMIR지속성동통모형,응용von Frey섬유세사법화Hargreaves법측정술전급술후제1、3、7、12、22、32천적궤계축족반사역치(mechanical withdrawal threshold,MWT)화열축족잠복기(thermalwithdrawallatency,TWL);병차매조채용수궤수자표법수궤취4지우술후12d시전경하관찰수술측은신경손상정황.결과 여공백대조조,모형조대서MWT재술후제1천즉출현강저(P=0.015＜0.05);12 d시하강최위현저(P=0.001＜0.01),32d시차이무통계학의의(P=0.772＞0.05);이가수술조여공백대조조비교재각시간점MWT차이무통계학의의;정개관찰시간과정중모형조대서TWL균무명현개변;SMIR술급가수술후은신경균미출현수초종창혹탈수초등신경손상현상.결론 진일보증실료SMIR모형적은정성화가중복성,장위연구술후지속성동통적궤제급치료조시제공일개신적평태.
Objective To establish a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR)and to test its efficacy. Methods Thirty-six male SD rats were randomly divided into three groups (n= 12): control group, sham group and model group. SMIR was produced as Flatters described and the threshold with von Frey filament and radiant heat stimulation were measured before operation (baseline) and at 1st, 3rd, 7th, 12th, 22nd and 32nd postoperative day. The structural damages to myelin sheath of saphenous nerve in 4 rats of each group randomly were observed with electron microscope at 12th postoperative day. Results SMIR surgery induced signi?cantly persistent mechanical hypersensitivity to von Frey stimulation of the plantar ipsilateral hindpaw. Compared to the responses of control group rats, the threshold to mechanical stimuli started to decrease at 1 d (P=0.015＜0.05) and peaked at 12 d (P=0.001＜0.01) after operation. There was no significant difference between the SMIR group and control group at 32 d after surgery (P=0.772＞0.05). Mechanical sensitivity of control group and sham group did not significantly change. SMIR did not induce significant heat hyperalgesia. Electron microscopy of saphenous nerve sections did not show demyelination or oedema in the saphenous nerve of the model group or sham group. Conclusion The results of the present study suggest that the model of persistent postoperative pain evoked by SMIR is stable and repeatable, which may provide a new stage for the study on the mechanisms and treatment of postoperative pain.
