国际放射医学核医学杂志
國際放射醫學覈醫學雜誌
국제방사의학핵의학잡지
INTERNATIONAL JOURNAL OF RADIATION MEDICINE AND NUCLEAR MEDICINE
2010年
1期
11-15
,共5页
冼伟均%冯彦林%袁建伟%张宁%黄克敏%贺小红%袁白虹%苏少弟%刘艳%叶秋莲
冼偉均%馮彥林%袁建偉%張寧%黃剋敏%賀小紅%袁白虹%囌少弟%劉豔%葉鞦蓮
승위균%풍언림%원건위%장저%황극민%하소홍%원백홍%소소제%류염%협추련
鼻咽肿瘤%小鼠,裸%X线疗法%氟脱氧葡萄糖F18%正电子发射断层显像术%体层摄影术,X线计算机%Ki-67,抗原
鼻嚥腫瘤%小鼠,裸%X線療法%氟脫氧葡萄糖F18%正電子髮射斷層顯像術%體層攝影術,X線計算機%Ki-67,抗原
비인종류%소서,라%X선요법%불탈양포도당F18%정전자발사단층현상술%체층섭영술,X선계산궤%Ki-67,항원
Nasopharyngeal neoplasms%Mice,nude%X-ray therapy%Fluorodeoxyglucose F18%Positron-emission tomography%Tomography,X-ray computed%Ki-67,Antigen
目的 探讨18FDG PET-CT早期评估裸鼠鼻咽癌移植瘤放疗疗效的价值,并初步分析18F-FDG PET-CT与肿瘤细胞增殖因子Ki67表达之间的关系,为临床进一步研究提供依据.方法 将15只鼻咽癌裸鼠移植瘤动物模型随机分成3组:每组5只,对照组:行18F-FDG PET-CT后并处死;6 Gy放疗组:于放疗前及放疗后1d分别给予显像后处死;12 Gy放疗组:于放疗前、放疗6Gv时、放疔12Gv后第2 d及6 d时分别行18F-FDGPET-CT,并于最后一次显像后处死.所有裸鼠处死后取移植瘤行病理学检查及Ki67染色,分析放疗疗效与Ki67表达的相关性.结果 ①12 Gy放疗组放疗前平均T/NT值为2.17±0.31,放疗6 Gy时平均T/NT值为1.68±0.42,放疗12 Gy后2 d时平均T/NT值为1.41±0.40,放疗12 Gy后6 d时平均T/NT值为0.70±0.12,放疗前与放疗6 Gy时没有统计学差异(t=2.108,P>0.05);放疗12 Gy后2 d与放疗前、放疗6 Cy时比较明显减低,有统计学差异(与放疗前比较,t=2.80,P<0.05;与放疗6 Gy 时比较,t=3.14,P<0.05);放疗12 Gy后6 d移植瘤的T/NT值较放疗后2 d进一步减低(与放疗后2 d比较,t=3.49,P<0.05;与放疗前比较,t=8.01,P<0.01).②鼻咽癌移植瘤Ki67的表达随放疗剂量的增加明显降低,不同组别间Ki67的表达有明显差别(F=21.95,P<0.01):12 Gy放疗组Ki67表达显著低于对照组(t=7.145,P<0.01)及6 Gy放疗组(t=2.384,P<0.05);6 Cy放疗组Ki67的表达较对照组减低同样存在统计学意义(t=4.320,P<0.01).③放疗前移植瘤T/NT值与Ki67表达之间无相关性.④放疗过程中移植瘤T/NT值的变化与Ki67表达水平呈正相关(r=0.532,P<0.05).结论 18F-FDG PET-CT对早期评估裸鼠鼻咽癌移植瘤的放疗疗效具有重要价值,放疗后6d是一个合适的时间点;18F-FDG PET-CT结合Ki67的表达变化可以反映鼻咽癌的放疗敏感性.
目的 探討18FDG PET-CT早期評估裸鼠鼻嚥癌移植瘤放療療效的價值,併初步分析18F-FDG PET-CT與腫瘤細胞增殖因子Ki67錶達之間的關繫,為臨床進一步研究提供依據.方法 將15隻鼻嚥癌裸鼠移植瘤動物模型隨機分成3組:每組5隻,對照組:行18F-FDG PET-CT後併處死;6 Gy放療組:于放療前及放療後1d分彆給予顯像後處死;12 Gy放療組:于放療前、放療6Gv時、放疔12Gv後第2 d及6 d時分彆行18F-FDGPET-CT,併于最後一次顯像後處死.所有裸鼠處死後取移植瘤行病理學檢查及Ki67染色,分析放療療效與Ki67錶達的相關性.結果 ①12 Gy放療組放療前平均T/NT值為2.17±0.31,放療6 Gy時平均T/NT值為1.68±0.42,放療12 Gy後2 d時平均T/NT值為1.41±0.40,放療12 Gy後6 d時平均T/NT值為0.70±0.12,放療前與放療6 Gy時沒有統計學差異(t=2.108,P>0.05);放療12 Gy後2 d與放療前、放療6 Cy時比較明顯減低,有統計學差異(與放療前比較,t=2.80,P<0.05;與放療6 Gy 時比較,t=3.14,P<0.05);放療12 Gy後6 d移植瘤的T/NT值較放療後2 d進一步減低(與放療後2 d比較,t=3.49,P<0.05;與放療前比較,t=8.01,P<0.01).②鼻嚥癌移植瘤Ki67的錶達隨放療劑量的增加明顯降低,不同組彆間Ki67的錶達有明顯差彆(F=21.95,P<0.01):12 Gy放療組Ki67錶達顯著低于對照組(t=7.145,P<0.01)及6 Gy放療組(t=2.384,P<0.05);6 Cy放療組Ki67的錶達較對照組減低同樣存在統計學意義(t=4.320,P<0.01).③放療前移植瘤T/NT值與Ki67錶達之間無相關性.④放療過程中移植瘤T/NT值的變化與Ki67錶達水平呈正相關(r=0.532,P<0.05).結論 18F-FDG PET-CT對早期評估裸鼠鼻嚥癌移植瘤的放療療效具有重要價值,放療後6d是一箇閤適的時間點;18F-FDG PET-CT結閤Ki67的錶達變化可以反映鼻嚥癌的放療敏感性.
목적 탐토18FDG PET-CT조기평고라서비인암이식류방료료효적개치,병초보분석18F-FDG PET-CT여종류세포증식인자Ki67표체지간적관계,위림상진일보연구제공의거.방법 장15지비인암라서이식류동물모형수궤분성3조:매조5지,대조조:행18F-FDG PET-CT후병처사;6 Gy방료조:우방료전급방료후1d분별급여현상후처사;12 Gy방료조:우방료전、방료6Gv시、방정12Gv후제2 d급6 d시분별행18F-FDGPET-CT,병우최후일차현상후처사.소유라서처사후취이식류행병이학검사급Ki67염색,분석방료료효여Ki67표체적상관성.결과 ①12 Gy방료조방료전평균T/NT치위2.17±0.31,방료6 Gy시평균T/NT치위1.68±0.42,방료12 Gy후2 d시평균T/NT치위1.41±0.40,방료12 Gy후6 d시평균T/NT치위0.70±0.12,방료전여방료6 Gy시몰유통계학차이(t=2.108,P>0.05);방료12 Gy후2 d여방료전、방료6 Cy시비교명현감저,유통계학차이(여방료전비교,t=2.80,P<0.05;여방료6 Gy 시비교,t=3.14,P<0.05);방료12 Gy후6 d이식류적T/NT치교방료후2 d진일보감저(여방료후2 d비교,t=3.49,P<0.05;여방료전비교,t=8.01,P<0.01).②비인암이식류Ki67적표체수방료제량적증가명현강저,불동조별간Ki67적표체유명현차별(F=21.95,P<0.01):12 Gy방료조Ki67표체현저저우대조조(t=7.145,P<0.01)급6 Gy방료조(t=2.384,P<0.05);6 Cy방료조Ki67적표체교대조조감저동양존재통계학의의(t=4.320,P<0.01).③방료전이식류T/NT치여Ki67표체지간무상관성.④방료과정중이식류T/NT치적변화여Ki67표체수평정정상관(r=0.532,P<0.05).결론 18F-FDG PET-CT대조기평고라서비인암이식류적방료료효구유중요개치,방료후6d시일개합괄적시간점;18F-FDG PET-CT결합Ki67적표체변화가이반영비인암적방료민감성.
Objective To investigate the value of 18F-FDG PET-CT scans on early assessing the therapeutic effects on human nasopharyngeal carcinoma (NPC) xenograft in nude mice after radiotherapy,and preliminarily analyze the relation of 18F-FDG PET-CT scans and the positive expression rate of Ki67, and provide a basis for further clinical studies. Methods Fifteen xenograft nude mice of NPC were randomly divided into three groups (5 nude mice per group). Control group: all mice immediately executed after the 18F-FDG PET-CT scans and taken pathology; 6 Gy radiotherapy group: all mice took 18F-FDG PET-CT scans before and 1 d after receiving 6 Gy irradiation, and were killed immediately to take pathology; 12 Gy radiotherapy group: all mice received 12 Gy irradiation (6 Gy each time, totally 2 times). They took 18F-FDG PET-CT scans before radiotherapy, after receiving the first 6 Gy irradiation, 2 d and 6 d after receiving 12 Gy irradiation respectively ,and then were executed to take pathology after the last scan. The expression of Ki67 in xenografts of different groups was detected and the relation between their changes and T/NT were analyzed.Results ①For 12 Gy radiotherapy group, the average T/NT ratios of the NPC xenograft of pre-radiotherapy,
after the first 6 Gy irradiation, 2 d and 6 d after 12 Gy radiotherapy were 2.17±0.31, 1.68±0.42, 1.41±0.40,and 0.70±0.12, respectively. The average T/NT ratios were not statistically difference before and after the first 6 Gy irradiation. The average T/NT ratio of 2 d after radiotherapy decreased significantly than pre-radiotherapy (t=2.80, P<0.05) and after the first 6 Gy irradiation (t=3.14, P<0.05); The average T/NT ratio of 6d after radiotherapy was decreased seriously than 2 d after radiotherapy(t=3.49, P<0.05, compared with preradiotherapy t=8.01, P<0.01). ② The positive expression rates of Ki67 of NPC xenograft markedly decreased after receiving irradiation. There were significant differences in the positive expressions of Ki67 between different group s(F=21.95, P<0.01).The positive expression of Ki67 in the 12 Gy radiotherapy group decreased significantly compared with the control group (t=7.145, P<0.01) and the 6 Gy radiotherapy group (t=2.384, P<0.05). The positive expression of Ki67 in the 6 Gy radiotherapy group was less than the control group, also have statistical significance(t=4.320, P<0.01).③There was no correlation between the T/NT ratio of NPC xenograft and the positive expression of Ki67. ④Positive correlation was found between the variance of T/NT ratio and the variance of the positive expression of Ki67 of NPC xenograft (r=0.532, P<0.05).Conclusions 18F-FDG PET-CT imaging has an important value on early assessing the therapeutic effects of NPC xenograft in nude mice after radiotherapy, and the 6th day after radiotherapy is an appropriate time point.18F-FDG PET-CT imaging can reflect the variance of the positive expression of Ki67 of NPC, and yet indirectly reflect the sensitivity of NPC to radiotheraphy.
