中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
1期
9-14
,共6页
聂琦%刘璐%刘志勇%黄培林%兰兴昊%高海林%吴清华%孙晋%黄鹰
聶琦%劉璐%劉誌勇%黃培林%蘭興昊%高海林%吳清華%孫晉%黃鷹
섭기%류로%류지용%황배림%란흥호%고해림%오청화%손진%황응
胶体~(32)P-磷酸铬%间质%动物%安全性
膠體~(32)P-燐痠鉻%間質%動物%安全性
효체~(32)P-린산락%간질%동물%안전성
~(32) P-colloidal chromic phosphate%Interstitial%Experimental animal%Security
目的 探讨胶体~(32)P-磷酸铬(~(32)P-CP)在正常Beagle犬的肝叶或臀大肌行间质注射的安全性.方法 10只Beagle犬,随机分成间质给药不同剂量(185和370 MBq)、不同部位(臀大肌和肝脏)及冷胶体对照5组(n＝2).术后不同时间点称量体重,行血液生化学检查,ECT轫致辐射显像,组织形态学动态观察及连续测量体表、血液、尿液和粪便放射性计数率值.计量数据以均数±标准差((x)±s)表示,采用SPSS 13.0软件进行统计分析.结果 给药后ECT示肝脏组全肝显影,放射性分布呈团块状不均匀,肌肉组局部放射性持续浓聚,肝脏未见显影.术后第4组犬体重进行性减少,45 d时较术前减少2.7 kg,余组体重增值均数依序为3.0、1.6、0.8和3.1 kg.第4组血小板、红细胞术后有明显减少.分别于给药后23和45 d死亡,死亡前谷草转氨酶和谷丙转氨酶均有急剧升高;其余组间血液和血生化学差异无统计学意义.术后体表分区测定以注射部位放射性计数率值为最高,其次为膀胱、脾.肝脏组血液峰时为5 rain,峰值分别为0.5×10~7/min和1.0×10~7/min;肌肉组持续在3×10~5/min左右.组织学表现肌肉组和肝脏185 MBq组4周内有充血水肿改变,8周后组织结构恢复正常;肝脏370 MBq组4周内部分肝细胞坏死,6周时见大量肝细胞气球样变,充血水肿明显,肝小叶结构不清.尿液、粪便中放射性计数率肌肉组峰时均数分别为13和12 d,峰值为(42.0 ±3.3)x 10~4/min和(29.6±4.5)×10~4/min;肝脏组峰时为5和9 d,峰值为(49.0±10.2)×10~4/min和(28.5±7.1)×10~4/min.至30 d肌肉组从尿液和粪便中累积排泄率为36.58%和10.62%,肝脏组为23.48%和8.76%.吸收剂量肝脏组肝脏为(30.6 ±2.3)、(55.6±4.4)Gy;肌肉组肌肉注射部位为(53.4±3.1)、(98.1±3.3)Gy,肝脏为(2.3±1.3)、(6.5±1.2)Gy.结论 Beagle犬肝脏间质注射794.39 MBq/m~2,肝脏吸收剂量为56 Gy时有较强肝毒性及全身毒副作用,是其致死剂量.肌肉给药463.98-772.93 MBq/m~2是安全剂量范围.~(32)P-CP间质给药是适用于治疗凡穿刺所能到达的乏血供及中等血供实体瘤的安全手段.
目的 探討膠體~(32)P-燐痠鉻(~(32)P-CP)在正常Beagle犬的肝葉或臀大肌行間質註射的安全性.方法 10隻Beagle犬,隨機分成間質給藥不同劑量(185和370 MBq)、不同部位(臀大肌和肝髒)及冷膠體對照5組(n＝2).術後不同時間點稱量體重,行血液生化學檢查,ECT軔緻輻射顯像,組織形態學動態觀察及連續測量體錶、血液、尿液和糞便放射性計數率值.計量數據以均數±標準差((x)±s)錶示,採用SPSS 13.0軟件進行統計分析.結果 給藥後ECT示肝髒組全肝顯影,放射性分佈呈糰塊狀不均勻,肌肉組跼部放射性持續濃聚,肝髒未見顯影.術後第4組犬體重進行性減少,45 d時較術前減少2.7 kg,餘組體重增值均數依序為3.0、1.6、0.8和3.1 kg.第4組血小闆、紅細胞術後有明顯減少.分彆于給藥後23和45 d死亡,死亡前穀草轉氨酶和穀丙轉氨酶均有急劇升高;其餘組間血液和血生化學差異無統計學意義.術後體錶分區測定以註射部位放射性計數率值為最高,其次為膀胱、脾.肝髒組血液峰時為5 rain,峰值分彆為0.5×10~7/min和1.0×10~7/min;肌肉組持續在3×10~5/min左右.組織學錶現肌肉組和肝髒185 MBq組4週內有充血水腫改變,8週後組織結構恢複正常;肝髒370 MBq組4週內部分肝細胞壞死,6週時見大量肝細胞氣毬樣變,充血水腫明顯,肝小葉結構不清.尿液、糞便中放射性計數率肌肉組峰時均數分彆為13和12 d,峰值為(42.0 ±3.3)x 10~4/min和(29.6±4.5)×10~4/min;肝髒組峰時為5和9 d,峰值為(49.0±10.2)×10~4/min和(28.5±7.1)×10~4/min.至30 d肌肉組從尿液和糞便中纍積排洩率為36.58%和10.62%,肝髒組為23.48%和8.76%.吸收劑量肝髒組肝髒為(30.6 ±2.3)、(55.6±4.4)Gy;肌肉組肌肉註射部位為(53.4±3.1)、(98.1±3.3)Gy,肝髒為(2.3±1.3)、(6.5±1.2)Gy.結論 Beagle犬肝髒間質註射794.39 MBq/m~2,肝髒吸收劑量為56 Gy時有較彊肝毒性及全身毒副作用,是其緻死劑量.肌肉給藥463.98-772.93 MBq/m~2是安全劑量範圍.~(32)P-CP間質給藥是適用于治療凡穿刺所能到達的乏血供及中等血供實體瘤的安全手段.
목적 탐토효체~(32)P-린산락(~(32)P-CP)재정상Beagle견적간협혹둔대기행간질주사적안전성.방법 10지Beagle견,수궤분성간질급약불동제량(185화370 MBq)、불동부위(둔대기화간장)급랭효체대조5조(n＝2).술후불동시간점칭량체중,행혈액생화학검사,ECT인치복사현상,조직형태학동태관찰급련속측량체표、혈액、뇨액화분편방사성계수솔치.계량수거이균수±표준차((x)±s)표시,채용SPSS 13.0연건진행통계분석.결과 급약후ECT시간장조전간현영,방사성분포정단괴상불균균,기육조국부방사성지속농취,간장미견현영.술후제4조견체중진행성감소,45 d시교술전감소2.7 kg,여조체중증치균수의서위3.0、1.6、0.8화3.1 kg.제4조혈소판、홍세포술후유명현감소.분별우급약후23화45 d사망,사망전곡초전안매화곡병전안매균유급극승고;기여조간혈액화혈생화학차이무통계학의의.술후체표분구측정이주사부위방사성계수솔치위최고,기차위방광、비.간장조혈액봉시위5 rain,봉치분별위0.5×10~7/min화1.0×10~7/min;기육조지속재3×10~5/min좌우.조직학표현기육조화간장185 MBq조4주내유충혈수종개변,8주후조직결구회복정상;간장370 MBq조4주내부분간세포배사,6주시견대량간세포기구양변,충혈수종명현,간소협결구불청.뇨액、분편중방사성계수솔기육조봉시균수분별위13화12 d,봉치위(42.0 ±3.3)x 10~4/min화(29.6±4.5)×10~4/min;간장조봉시위5화9 d,봉치위(49.0±10.2)×10~4/min화(28.5±7.1)×10~4/min.지30 d기육조종뇨액화분편중루적배설솔위36.58%화10.62%,간장조위23.48%화8.76%.흡수제량간장조간장위(30.6 ±2.3)、(55.6±4.4)Gy;기육조기육주사부위위(53.4±3.1)、(98.1±3.3)Gy,간장위(2.3±1.3)、(6.5±1.2)Gy.결론 Beagle견간장간질주사794.39 MBq/m~2,간장흡수제량위56 Gy시유교강간독성급전신독부작용,시기치사제량.기육급약463.98-772.93 MBq/m~2시안전제량범위.~(32)P-CP간질급약시괄용우치료범천자소능도체적핍혈공급중등혈공실체류적안전수단.
Objective To explore the possibility and safety of ~(32)P-colloidal chromic phosphate interstitial injection.Methods Ten Beagle dogs were randomly divided into 5 groups (n ＝ 2) according to different doses (185 and 370 MBq) ,different sites (gluteus maximus and liver) and cold colloid as a control group.At different time-points after surgery,the weights of dogs were measured,and the blood and blood biochemical inspections were examined.ECT imaging was performed and histomorphology was observed dynamically.The radioactive counts of body surface for 90 days,blood for 12 weeks and urine and feces for 30 days were measured continuously.Measured data were expressed by mean ±standard error ((x) ±s) and SPSS 13.0 software was used for statistical analysis.Results ECT imaging demonstrated that the whole liver imaging was obtained although the radioactive distribution was uneven in liver groups,and the radioactivity concentrated continuously in the area of injection,but no liver imaging in muscle groups.Dogs in group 4 lost weight progressively and reduced by 2.7 kg till 45 d after operation.While the mean weight increments in the other groups were 3.0,1.6,0.8 and 3.1 kg in order.In group 4,PLT and RBC reduced obviously.Dogs died at 23 or 45 d.AST and ALT were elevated sharply before death.In the other groups,blood and blood biochemistry inspection showed there were no significant statistical differences.The highest radioactive counts after operation were obtained from the injection spot,while the urinary bladder and the spleen were followed.The peak of blood cpm in liver groups presented at 5 min.Peak values were 0.5 × 10~7/min and 1.0 × 10~7/min,respectively.The blood cpm in the muscle groups was always maintained at 3 × l0~5/min.Histology study showed the hyperemia dropsy changes in muscle groups and 185 MBq liver group in 4 weeks,while after 8 weeks the organizational structure restored normally.There were partial liver cells necrosis in 4 weeks,and the massive liver cells balloon type changes in 6 weeks,as well as obvious hyperemia dropsy and the hepatic lobe structure unclear in 370 MBq liver group.For the radioactive counts of urine and excrement,the peak appeared at 13 and 12 d respectively,and the peak values were (42.0 ±3.3) × 10~4 and (29.6 ±4.5) ×10~4 /min in muscle groups,respectively ;while the peak appeared at 5 and 9 d,respectively and the peak values were (49.0 ± 10.2) × 10~4 and (28.5 ± 7.1) × 10~4 /min in liver groups,respectively.Cumulative excretion ratios in urine and excrement were 36.58% and 10.62% in muscle groups,respectively and 23.48% and 8.76% in liver groups till 30 d,respectively.The liver absorbed doses were 30.6 and 55.6 Gy in liver groups,while those were 2.3 and 6.5 Gy in muscle groups.The maximus absorbed doses of gluteus were 53.4 and 98.1 Gy in muscle groups.Conclusions When ~(32)P- colloidal chromic phosphate of 794.39 MBq/m~2 was injected into the liver of Beagle,the liver absorbed dose was 56 Gy,which could be lethal dose for its strong liver toxicity and systemic side effects.Injection of 463.98-772.93 MBq/m~2 in muscle of Beagle could be safe.~(32) P-colloidal chromic phosphate interstitial injection is secure to treat the solid tumors with poor and middle blood supply which could be reached by puncture.
