CAJ | 학술논문

以OKT8、OKT4、OKT9系列单克隆抗体作为检测试剂,采用改良的8ACE-Ig花环法对20例EHF患者末梢血T细胞亚群进行了动态检测.结果发现0KT+8 细胞百分比于发热期即明显增高,达37.67±7.34.休克少尿期升至41.79±9.28,达最高值.多尿期开始下降,于恢复期降至正常水平.OKT+4 细胞百分比除休克少尿期降低(23.79±4.39)外,余基本在正常范围.与此变化相应的是在病程中OKT4/OKT8比值下降,于休克少尿期明显倒置(0.60±0.19).结合EHF患者有体液免疫功能亢进,OKT+8 细胞百分比的升高可能主要是Tc细胞增高所致T细胞介导的杀伤作用在EHF的发病机理中可能起一定的作用.
이OKT8、OKT4、OKT9계렬단극륭항체작위검측시제,채용개량적8ACE-Ig화배법대20례EHF환자말소혈T세포아군진행료동태검측.결과발현0KT+8 세포백분비우발열기즉명현증고,체37.67±7.34.휴극소뇨기승지41.79±9.28,체최고치.다뇨기개시하강,우회복기강지정상수평.OKT+4 세포백분비제휴극소뇨기강저(23.79±4.39)외,여기본재정상범위.여차변화상응적시재병정중OKT4/OKT8비치하강,우휴극소뇨기명현도치(0.60±0.19).결합EHF환자유체액면역공능항진,OKT+8 세포백분비적승고가능주요시Tc세포증고소치T세포개도적살상작용재EHF적발병궤리중가능기일정적작용.
OKT monoclonal antibodies were used to deteot T cell subsets of peripheral blood in patients with epidemio hemorrhagic fever(EHF).The amount of OKT+8 cells began to increase in febrile phase(p<0.001) and reached its highest level in shook/oligurio phase.on began to decrease in diuretic phase and returned to normal in eonvalesoent phase.On the eontrary,OKT4/OKT8 ratio decreased in the febrile phase and reversed in the shook/otigurio phase.The peroentage of OKT+4 cells was normal in all phases but Shook/oligurio.phase.The decreased and reversed OKT4/OKT8 raio mainly resulted from the markedly increased OKT+8 cells.As the patients with EHF had humeral hyperimmunity,it is proposed that most of the increased 0KT+8 cells were cytotoxic T cells(To).Theincreased To were probably responsible for the widespread damage of systemio small blood vessels and various organs in patients with EHF.