中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2012年
4期
195-199
,共5页
王春莹%陈达之%刘俊%李春艳%林镯%阳韬%陆小蒟%陈永平
王春瑩%陳達之%劉俊%李春豔%林鐲%暘韜%陸小蒟%陳永平
왕춘형%진체지%류준%리춘염%림탁%양도%륙소구%진영평
骨形态发生蛋白7%果蝇蛋白质类%磷酰化%肌,平滑肌动蛋白%纤连蛋白类
骨形態髮生蛋白7%果蠅蛋白質類%燐酰化%肌,平滑肌動蛋白%纖連蛋白類
골형태발생단백7%과승단백질류%린선화%기,평활기동단백%섬련단백류
Bone morphogenetic proteins%Drosophila proteines%Phosphorylation%Muscle,smooth actins%Fiboronectins
目的 研究骨成形蛋白7 (BMP7)对大鼠肝星状细胞(HSC)增殖的抑制作用.方法 体外培养HSC-T6细胞,分为对照组、TGFβ1组、TGFβ1+ BMP7组和TGFβ1+ BMP7+ Noggin组.RT-PCR法检测果蝇蛋白质1(smad1)、α-平滑肌肌动蛋白(α-SMA)、gremlin和纤维连接蛋白(FN)mRNA的表达,Western印迹法检测磷酸化smad1/5/8(P-smad1/5/8)、α-SMA、gremlin蛋白的表达.采用单因素方差分析、LSD检验、Dunnett T3检验和Pearson直线相关分析进行统计学处理.结果 对照组HSC-T6细胞中可见P-smad1/5/8、α-SMA、gremlin及FN的表达,TGFβ1刺激后表达量明显增加,差异有统计学意义(均P＜0.05).与TGFβ1组比较,TGFβ1+ BMP7组α-SMA、gremlin及FN表达下调,但P-smad1/5/8蛋白表达增加(1.613±0.031比2.503±0.014;t=34.89,P＜0.05).TGFβ1+ BMP7+ Noggin组α-SMA、gremlin及FN表达较TGFβ1+ BMP7组增加,而P-smad1/5/8蛋白表达下调(t=34.05,P＜0.05).smadl mRNA在各组表达均无显著变化.结论 BMP7对α-SMA、gremlin和FN均有较强的抑制作用.
目的 研究骨成形蛋白7 (BMP7)對大鼠肝星狀細胞(HSC)增殖的抑製作用.方法 體外培養HSC-T6細胞,分為對照組、TGFβ1組、TGFβ1+ BMP7組和TGFβ1+ BMP7+ Noggin組.RT-PCR法檢測果蠅蛋白質1(smad1)、α-平滑肌肌動蛋白(α-SMA)、gremlin和纖維連接蛋白(FN)mRNA的錶達,Western印跡法檢測燐痠化smad1/5/8(P-smad1/5/8)、α-SMA、gremlin蛋白的錶達.採用單因素方差分析、LSD檢驗、Dunnett T3檢驗和Pearson直線相關分析進行統計學處理.結果 對照組HSC-T6細胞中可見P-smad1/5/8、α-SMA、gremlin及FN的錶達,TGFβ1刺激後錶達量明顯增加,差異有統計學意義(均P＜0.05).與TGFβ1組比較,TGFβ1+ BMP7組α-SMA、gremlin及FN錶達下調,但P-smad1/5/8蛋白錶達增加(1.613±0.031比2.503±0.014;t=34.89,P＜0.05).TGFβ1+ BMP7+ Noggin組α-SMA、gremlin及FN錶達較TGFβ1+ BMP7組增加,而P-smad1/5/8蛋白錶達下調(t=34.05,P＜0.05).smadl mRNA在各組錶達均無顯著變化.結論 BMP7對α-SMA、gremlin和FN均有較彊的抑製作用.
목적 연구골성형단백7 (BMP7)대대서간성상세포(HSC)증식적억제작용.방법 체외배양HSC-T6세포,분위대조조、TGFβ1조、TGFβ1+ BMP7조화TGFβ1+ BMP7+ Noggin조.RT-PCR법검측과승단백질1(smad1)、α-평활기기동단백(α-SMA)、gremlin화섬유련접단백(FN)mRNA적표체,Western인적법검측린산화smad1/5/8(P-smad1/5/8)、α-SMA、gremlin단백적표체.채용단인소방차분석、LSD검험、Dunnett T3검험화Pearson직선상관분석진행통계학처리.결과 대조조HSC-T6세포중가견P-smad1/5/8、α-SMA、gremlin급FN적표체,TGFβ1자격후표체량명현증가,차이유통계학의의(균P＜0.05).여TGFβ1조비교,TGFβ1+ BMP7조α-SMA、gremlin급FN표체하조,단P-smad1/5/8단백표체증가(1.613±0.031비2.503±0.014;t=34.89,P＜0.05).TGFβ1+ BMP7+ Noggin조α-SMA、gremlin급FN표체교TGFβ1+ BMP7조증가,이P-smad1/5/8단백표체하조(t=34.05,P＜0.05).smadl mRNA재각조표체균무현저변화.결론 BMP7대α-SMA、gremlin화FN균유교강적억제작용.
Objective To investigate the inhibitory effect of bone morphogenetic protein 7 (BMP7) on proliferation of rat hepatic stellate cells (HSC).Methods HSC-T6 cells were cultured in vitro,and divided into four groups:control group,transforming growth factor (TGF) β1 group,TGFβ1 + BMP7 group,and TGFβ1 + BMP7 + Noggin group. The mRNA expressions of smad1,α-smooth muscle actin (α-SMA),gremlin and fibronectin (FN) were determined by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). The protein expressions of phosphorylated-smad1/5/8 (P-smad1/5/8),α-SMA and gremlin were detected by Western blot.The statistical analysis were done using one-factor analysis of variance,LSD-t test,Dunnett's T3 test and Pearson linear correlation analysis.Results Phosphorylated-smad1/5/8,α-SMA,gremlin and FN were expressed on HSC-T6 cells in control group,which were significantly up-regulated after TGFβ1 stimulation (P＜0.05).The expressions of α-SMA,gremlin and FN were significantly down-regulated in TGFβ1 + BMP7 group compared with TGFβ1 group, while P-smad1/5/8 expression was upregulated (1.613±0.031 vs.2.503±0.014; t=34.89,P＜0.05).The expressions of α-SMA,gremlin and FN in TGFβ1 + BMP7 + Noggin group were up-regulated than those in TGFβ1 + BMP7 group,while P-smad1/5/8 was down-regulated (t=34.05,P＜0.05).There was no difference of total smad1 mRNA expression among each group.Conclusion These results collectively suggest that BMP7 strongly inhibits the expressions of α-SMA,gremlin and FN.
