国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2012年
5期
317-319,360
,共4页
王玲玲%徐世元%雷洪伊%吴廷丽%范凤飞
王玲玲%徐世元%雷洪伊%吳廷麗%範鳳飛
왕령령%서세원%뢰홍이%오정려%범봉비
罗哌卡因%惊厥%学习记忆%突触后致密物蛋白
囉哌卡因%驚厥%學習記憶%突觸後緻密物蛋白
라고잡인%량궐%학습기억%돌촉후치밀물단백
Ropivacaine%Convulsion%Learning and memory%Post synaptic density-95 protein
目的 通过制备幼鼠罗哌卡因毒性惊厥模型,观察其对幼鼠成年后学习记忆能力和突触后致密物蛋白-95( post synaptic density-95protein,PSD-95)表达的影响.方法 选用18日龄Sprague -Dawley (SD)幼鼠20只,采用随机数字表法分为罗哌卡因组(R组)和生理盐水组(N组)2组,每组10只.连续水迷宫训练3d,每天2次,达到标准者纳入实验(潜伏期≤120 s).于21日龄分别经腹腔单次注射罗哌卡因(33.8 mg/kg)和生理盐水(0.1 ml/kg).出现惊厥鼠纳入实验,常规饲养至第55日龄再次行水迷宫测试.前3d为训练学习阶段,每天训练两次.第4天测试记忆能力.测试结束后选取5只取海马组织,采用Weston Blot技术行PSD-95含量测定.另两组各取5只,取右侧海马CA1区于电镜下观察突触后致密物厚度(post synaptic density,PSD)变化.结果 R组和N组水迷宫测试第1天潜伏期分别为(12.6±3.4)、(10.5±4.1)s；第2天潜伏期分别为(6.8±2.8)、(7.1±1.3)s；第3天潜伏期分别为(6.2±0.7)、(6.4±1.1)s；第4天潜伏期分别为(5.0±2.7)、(8.1±4.0)s；训练阶段和第60日龄潜伏期之间差异均无统计学意义(P＞0.05).R组和N组PSD-95蛋白含量基础值和60日龄分别为(0.60±0.08)、(0.61±0.05),两组差异无统计学意义(P＞0.05).R组和N组60日龄PSD厚度分别为(84±6)、(81±7) nm,两组差异无统计学意义(P＞0.05).结论 幼年期单次罗哌卡因毒性惊厥对成年后鼠学习记忆、PSD-95蛋白及PSD厚度无明显影响.
目的 通過製備幼鼠囉哌卡因毒性驚厥模型,觀察其對幼鼠成年後學習記憶能力和突觸後緻密物蛋白-95( post synaptic density-95protein,PSD-95)錶達的影響.方法 選用18日齡Sprague -Dawley (SD)幼鼠20隻,採用隨機數字錶法分為囉哌卡因組(R組)和生理鹽水組(N組)2組,每組10隻.連續水迷宮訓練3d,每天2次,達到標準者納入實驗(潛伏期≤120 s).于21日齡分彆經腹腔單次註射囉哌卡因(33.8 mg/kg)和生理鹽水(0.1 ml/kg).齣現驚厥鼠納入實驗,常規飼養至第55日齡再次行水迷宮測試.前3d為訓練學習階段,每天訓練兩次.第4天測試記憶能力.測試結束後選取5隻取海馬組織,採用Weston Blot技術行PSD-95含量測定.另兩組各取5隻,取右側海馬CA1區于電鏡下觀察突觸後緻密物厚度(post synaptic density,PSD)變化.結果 R組和N組水迷宮測試第1天潛伏期分彆為(12.6±3.4)、(10.5±4.1)s；第2天潛伏期分彆為(6.8±2.8)、(7.1±1.3)s；第3天潛伏期分彆為(6.2±0.7)、(6.4±1.1)s；第4天潛伏期分彆為(5.0±2.7)、(8.1±4.0)s；訓練階段和第60日齡潛伏期之間差異均無統計學意義(P＞0.05).R組和N組PSD-95蛋白含量基礎值和60日齡分彆為(0.60±0.08)、(0.61±0.05),兩組差異無統計學意義(P＞0.05).R組和N組60日齡PSD厚度分彆為(84±6)、(81±7) nm,兩組差異無統計學意義(P＞0.05).結論 幼年期單次囉哌卡因毒性驚厥對成年後鼠學習記憶、PSD-95蛋白及PSD厚度無明顯影響.
목적 통과제비유서라고잡인독성량궐모형,관찰기대유서성년후학습기억능력화돌촉후치밀물단백-95( post synaptic density-95protein,PSD-95)표체적영향.방법 선용18일령Sprague -Dawley (SD)유서20지,채용수궤수자표법분위라고잡인조(R조)화생리염수조(N조)2조,매조10지.련속수미궁훈련3d,매천2차,체도표준자납입실험(잠복기≤120 s).우21일령분별경복강단차주사라고잡인(33.8 mg/kg)화생리염수(0.1 ml/kg).출현량궐서납입실험,상규사양지제55일령재차행수미궁측시.전3d위훈련학습계단,매천훈련량차.제4천측시기억능력.측시결속후선취5지취해마조직,채용Weston Blot기술행PSD-95함량측정.령량조각취5지,취우측해마CA1구우전경하관찰돌촉후치밀물후도(post synaptic density,PSD)변화.결과 R조화N조수미궁측시제1천잠복기분별위(12.6±3.4)、(10.5±4.1)s；제2천잠복기분별위(6.8±2.8)、(7.1±1.3)s；제3천잠복기분별위(6.2±0.7)、(6.4±1.1)s；제4천잠복기분별위(5.0±2.7)、(8.1±4.0)s；훈련계단화제60일령잠복기지간차이균무통계학의의(P＞0.05).R조화N조PSD-95단백함량기출치화60일령분별위(0.60±0.08)、(0.61±0.05),량조차이무통계학의의(P＞0.05).R조화N조60일령PSD후도분별위(84±6)、(81±7) nm,량조차이무통계학의의(P＞0.05).결론 유년기단차라고잡인독성량궐대성년후서학습기억、PSD-95단백급PSD후도무명현영향.
Objective To investigate the effect of juvenile convulsion induced by ropivacaine toxicity on adult learing,memory and post synaptic density-95protein(PSD-95) protein expression in rats. Methods Twenty Sprague-Dawley(SD)rats(age=18 d) were randomly divided into ropivacaine group(R group) and saline group(N group)(n=10).Morris water maze was performed on rats in the both groups for 3 days,2 times per day,rats fulfilling the standards were included in the experiments (latency period ≤ 120 s).Rats in the R group were intraperitoneally injected with 33.8 mg/kg ropivacaine,and those in the N group were injected with 0.1 ml/kg saline when rats were 21days old.The rats convulsed were adopted and performed with morris water maze again as before when they were 55 days old,4 days later memory test was performed.The sections of hippocampus were collected at the end of test,PSD-95 expression and post synaptic density(PSD) thickness was detected by Weston Blot and electron microscope,respectively(n=5). Results There was no significant differences in latency between the R group and the N group[d1(12.6±3.4) vs (10.5±4.1) s,d2 (6.8±2.8) vs (7.1±1.3) s,d3 (6.2±0.7) vs (6.4±1.1) s,d4 (5.0±2.7) vs (8.1±4.0) s respectively(P＞0.05)].There was no significant differences in PSD-95(0.6±0.1 ) vs (0.6±0.0) respectively(P＞0.05)and PSD(84±6),(81±7) nm,respectively(P＞0.05)between the R group and the N group. Conclusions There was no significant effect of juvenile convulsion induced by ropivacaine toxicity on adult learing,memory,and PSD-95 in rats.
