CAJ | 학술논문

目的了解食管基底细胞样鳞状细胞癌(BSC)的生物学特点。方法食管基底细胞样鳞状细胞癌8例，用CK4，CK18，CK19，EMA，CEA，α-SMA，S-100，LN，Col-Ⅳ， NSE，PCNA，p53等12种抗体进行免疫组化常规ABC法检测。结果 BSC的两种细胞成分，即基底样细胞(BC)和鳞状细胞(SC)对上述抗体反应截然不同。绝大部分病例(7例)BC专一地对CK19抗体呈阳性反应，而对其它4种上皮性抗体呈阴性反应；有4例BC对α-SMA 及S-100肌性抗体呈阳性；BC巢内玻璃样物对LN及Col-Ⅳ呈阳性反应；BC有较高的PCNA指数，平均为 54％。相反，7例SC成分均对CK4、CEA、EMA等上皮性抗体呈阳性；对CK19、α-SMA、S-100呈阴性； PCNA指数较低，平均为25％。结论食管BSC为多向分化的高度恶性肿瘤。BC代表向基底细胞和肌上皮方向分化，其增殖活性强；而 SC代表向典型的鳞状细胞分化。
목적료해식관기저세포양린상세포암(BSC)적생물학특점。 방법식관기저세포양린상세포암8례，용CK4，CK18，CK19，EMA，CEA，α-SMA，S-100，LN，Col-Ⅳ， NSE，PCNA，p53등12충항체진행면역조화상규ABC법검측。 결과 BSC적량충세포성분，즉기저양세포(BC)화린상세포(SC)대상술항체반응절연불동。절대부분병 례(7례)BC전일지대CK19항체정양성반응，이대기타4충상피성항체정음성반응；유4례BC대α-SMA 급S-100기성항체정양성；BC소내파리양물대LN급Col-Ⅳ정양성반응；BC유교고적PCNA지수，평균위 54％。상반，7례SC성분균대CK4、CEA、EMA등상피성항체정양성；대CK19、α-SMA、S-100정음성； PCNA지수교저，평균위25％。 결론식관BSC위다향분화적고도악성종류。BC대표향기저세포화기상피방향분화，기증식활성강；이 SC대표향전형적린상세포분화。
Objective To explore the biological features of basaloid squamous cell carcinoma (BSC) of the esophagus. Methods Cytokeratins (CK4, CK18 and CK19), epithelial membrane antigen (EMA), carcino embryo antigen (CEA), α-smooth muscle antigen (α-SMA), S-100, laminin (LN), collagen Ⅳ (Col-Ⅳ), neural specific enolase (NSE), proliferating cell nuclear antigen (PCNA) and p53 antibodies were used to detect the corresponding antigen expression in 8 cases of BSC with ABC immunohistochemical methods. Results Two kinds of BSC cell components have different responses to the above antibodies. For basaloid cells (BCs), 7 cases were positive for CK19, and were negative for the other 4 epithelial antibodies CK4, CK18, CEA and EMA. BCs of 4 cases were positive to the muscular antibodies α-SMA and S-100, and the hyaline degeneration in the tumor nests was positive for LN and Col-Ⅳ. BCs had a high index of PCNA, with an average level of 54%. For squamous cells (SCs), 7 cases were positive for the epithelial antigen CK4, CEA and EMA, but were negative for CK19, α-SMA and S-100. The index of PCNA of SC was low, with an average level of 25%. Conclusion BSC of the esophagus is a high-malignancy tumor which is of multi-oriented differentiation. BCs represent basal cells which have the tendency of myoepithelial differentiation and have strong proliferation ability, whereas SCs represent typical squamous cell differentiation.