CAJ | 학술논문

对高效木糖乳酸菌Lt的发酵特性进行了研究,同时对其发酵玉米芯L-乳酸进行了初步探讨.Lt菌株生长需要氧气,延迟期约为6 h,16 h以后进入稳定期,可利用木糖、葡萄糖、D-甘露糖、L-阿拉伯糖产L-乳酸,转化率为88.0%-98.7%.最适发酵温度为46℃,从46-52℃产酸量均较高.用6%的纯木糖发酵48 h,L-乳酸产量为57.35 g/L,转化率约为95%;用含6%还原糖的玉米芯磷酸水解液发酵48 h,L-乳酸产量为31.30 g/L.该菌株具有发酵温度高、适温范围宽、发酵时间短、转化率高、L-乳酸纯度高等优点.
대고효목당유산균Lt적발효특성진행료연구,동시대기발효옥미심L-유산진행료초보탐토.Lt균주생장수요양기,연지기약위6 h,16 h이후진입은정기,가이용목당、포도당、D-감로당、L-아랍백당산L-유산,전화솔위88.0%-98.7%.최괄발효온도위46℃,종46-52℃산산량균교고.용6%적순목당발효48 h,L-유산산량위57.35 g/L,전화솔약위95%;용함6%환원당적옥미심린산수해액발효48 h,L-유산산량위31.30 g/L.해균주구유발효온도고、괄온범위관、발효시간단、전화솔고、L-유산순도고등우점.
The growth of bacteria strain Lt takes oxygen,with the lag period of 6h and enters into the stationary growth phase at the 16th hour. Strain Lt can ferment xylose, glucose, D-mannose and L-arabinose into L-lactic acid with the conversion rate of 88.0%-98.7% , but failed with L-arabinose and lactose. The optimum fermentation me-mL medium in 250 mL bottle,inoculum size 10% ,shaker rev 160 r/min, at 46℃, inaerobically. Using pure xylose (60 g/L) as substrate, the L-lactic acid yield reached 57.35 g/L; using the corncob hydrolysate(reduced sugar 60 g/L) , the L-lactic acid yield reached 31.30 g/L.