CAJ | 학술논문

利用RAPD技术,对黑龙江省金丝桃属两种植物进行DNA分子遗传标记鉴别,从30种随机引物中筛选出,对乌腺金丝桃、长柱金丝桃扩增具有较明显多态性的3种引物,进而从基因水平探讨其遗传本质。结果表明：引物S75、S76、S80可作为乌腺金丝桃、长柱金丝桃的高特异性引物,用这三种鉴别引物对样品的DNA进行PCR反应,经琼脂糖凝胶电泳,就可以准确鉴别。
이용RAPD기술,대흑룡강성금사도속량충식물진행DNA분자유전표기감별,종30충수궤인물중사선출,대오선금사도、장주금사도확증구유교명현다태성적3충인물,진이종기인수평탐토기유전본질。결과표명：인물S75、S76、S80가작위오선금사도、장주금사도적고특이성인물,용저삼충감별인물대양품적DNA진행PCR반응,경경지당응효전영,취가이준학감별。
By the biological technique RAPD,We identified two species medicinal plants of Hypericum in Heilongjiang by DNA molecular genetic marker.From 30 random primers screened,the more obvious of H.attenuatum Choisy.,Hypericum.ascyron L.amplified polymorphic with three kinds of primers,and then from the gene level of their genetic nature.The results showed that： primers S75,S76,S80 can be used as H.attenuatum Choisy.,Hypericum ascyron L.of high-specific primers,using the three primer to identify the DNA samples for PCR reaction by agarose gel electrophoresis,can accurately identification.