CAJ | 학술논문

核糖体蛋白S6是核糖体蛋白家族的重要成员,在核糖体中发挥重要作用.利用农业部橡胶生物学重点开放实验室获得的部分序列设计引物,采用3′-RACE技术从巴西橡胶树胶乳中获得一个核糖体蛋白S6基因的完整阅读框,该基因编码249个氨基酸的多肽,含有一个典型的真核生物核糖体蛋白S6结构域.在HbRPS6氨基酸序列中没有预测到跨膜区和信号肽,二级结构分析表明:HbRPS6属于混合型蛋白.对10个RPS6系统进化分析表明:巴西橡胶树的RPS6基因在进化上具有保守性,与植物类玉米的RPS5基因亲缘关系最近,而与动物和酵母的亲缘关系相对较远.该基因的克隆和生物信息学分析为深入研究其功能奠定了基础.
핵당체단백S6시핵당체단백가족적중요성원,재핵당체중발휘중요작용.이용농업부상효생물학중점개방실험실획득적부분서렬설계인물,채용3′-RACE기술종파서상효수효유중획득일개핵당체단백S6기인적완정열독광,해기인편마249개안기산적다태,함유일개전형적진핵생물핵당체단백S6결구역.재HbRPS6안기산서렬중몰유예측도과막구화신호태,이급결구분석표명:HbRPS6속우혼합형단백.대10개RPS6계통진화분석표명:파서상효수적RPS6기인재진화상구유보수성,여식물류옥미적RPS5기인친연관계최근,이여동물화효모적친연관계상대교원.해기인적극륭화생물신식학분석위심입연구기공능전정료기출.
The ribosomal protein S6 is the key member of ribosomal protein family,and it plays an important role in the ribosomal proteins.The primers designed based on the known sequences acquired from Key Laboratory of Rubber Bidoye of Minisby of Agriculture,the gene was cloned with 3'-RACE technique from Hevea brasiliensis latex.The open reading frame encoded 249 amino acids,containing a conserved ribosomal S6 domain,so the gene was named as HbRPS6.No signal peptide and transmembrane structure were predicted within HbRPS6.The result of secondary structure predicted showed that the protein was classified as mixed type.The result of phylogenetic tree indicated that HbRPS6 displayed a high degree of sequence similarity to ZmRPS5,but not to animals and yeast.This paper will be benefit to further analyze the functions of HbRPS6.