CAJ | 학술논문

白玉菇液体发酵培养基的最优配方可为白玉菇工业扩大培养提供基础资料。以葡萄糖、酵母粉、KH2P04和MgSO4为主要因素，选择胞外多糖含量和菌丝体生物量为试验指标，采用L9（3^4）正交试验进行液体发酵培养基的优化。通过抽滤分离茵丝体，烘干称重，计算茵丝体生物量；用苯酚-硫酸法测定胞外多糖含量。试验测得通过对试验结果进行综合加权评分，确定了白玉菇液体培养基的最优配方为葡萄糖3％、酵母粉0．15％、KH2PO40．30％、MgSO40．20％。
백옥고액체발효배양기적최우배방가위백옥고공업확대배양제공기출자료。이포도당、효모분、KH2P04화MgSO4위주요인소，선택포외다당함량화균사체생물량위시험지표，채용L9（3^4）정교시험진행액체발효배양기적우화。통과추려분리인사체，홍간칭중，계산인사체생물량；용분분-류산법측정포외다당함량。시험측득통과대시험결과진행종합가권평분，학정료백옥고액체배양기적최우배방위포도당3％、효모분0．15％、KH2PO40．30％、MgSO40．20％。
The optimum liquid medium of white Hypsizigus marmoreus can provide the fundamental data for its extensive cultivation. We optimized fermentation medium with L9 （34） orthogonal test, the main ingredients of glucose, yeast powder, KH2PO4 and MgSO4, and exopolysaccharides content and mycelial biomass as test indexes. We also separated mycelia by extraction and filtering, dried and weighted them, and calculated their biomass. Phenol-sulfuric acid was then employed to determine the content of exopolysaccharides. Weighted evaluation of exopolysaccharides content and mycelial biomass shows that the optimum liquid medium is composed of glucose of 3%, yeast powder of 0. 15%, KH2PO4 of 0.30% and MgSO4 of 0.20%.