福建农林大学学报(自然科学版)
福建農林大學學報(自然科學版)
복건농림대학학보(자연과학판)
JOURNAL OF FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY(NATURAL SCIENCE EDITION)
2010年
1期
6-9
,共4页
高三基%陈平华%洪健%陈如凯
高三基%陳平華%洪健%陳如凱
고삼기%진평화%홍건%진여개
甘蔗黄叶病毒%叶片细胞%超微结构%细胞病理变化
甘蔗黃葉病毒%葉片細胞%超微結構%細胞病理變化
감자황협병독%협편세포%초미결구%세포병리변화
sugarcane yellow leaf virus%foliar cell%ultrastructure%cytopathological alteration
利用电镜技术对感染甘蔗黄叶病毒蔗叶组织超微结构进行观察表明,韧皮部伴胞及叶肉细胞内的线粒体、叶绿体等细胞器及细胞核都发生了明显的病理变化.线粒体形态异常,有的肿大、内嵴模糊,严重者内嵴消失,空泡化,仅剩未被消解的残骸;叶绿体被膜破裂,严重者被膜完全消解,基粒类囊体和基质片层消失,基质外流.维管束鞘细胞中的叶绿体被膜和基质片层也遭到破坏,淀粉粒增多、膨大;细胞核形态变为不规则,局部核膜破裂,核内染色质分布不均匀,呈降解状.
利用電鏡技術對感染甘蔗黃葉病毒蔗葉組織超微結構進行觀察錶明,韌皮部伴胞及葉肉細胞內的線粒體、葉綠體等細胞器及細胞覈都髮生瞭明顯的病理變化.線粒體形態異常,有的腫大、內嵴模糊,嚴重者內嵴消失,空泡化,僅剩未被消解的殘骸;葉綠體被膜破裂,嚴重者被膜完全消解,基粒類囊體和基質片層消失,基質外流.維管束鞘細胞中的葉綠體被膜和基質片層也遭到破壞,澱粉粒增多、膨大;細胞覈形態變為不規則,跼部覈膜破裂,覈內染色質分佈不均勻,呈降解狀.
이용전경기술대감염감자황협병독자협조직초미결구진행관찰표명,인피부반포급협육세포내적선립체、협록체등세포기급세포핵도발생료명현적병리변화.선립체형태이상,유적종대、내척모호,엄중자내척소실,공포화,부잉미피소해적잔해;협록체피막파렬,엄중자피막완전소해,기립류낭체화기질편층소실,기질외류.유관속초세포중적협록체피막화기질편층야조도파배,정분립증다、팽대;세포핵형태변위불규칙,국부핵막파렬,핵내염색질분포불균균,정강해상.
The ultrastructure alteration of diseased leaf infected sugarcane yellow leaf virus (SCYLV) was studied with electron microscopy. The results suggested that the ultrastructure alteration changed apparently in the mitochondria, chloroplast and nucleus of phloem companion cells and mesophyll cells. The abnormal mitochondria expanded with indistinct cristae. In serious abnormal mitochondria, cristae disappeared and vacuolation of mitochondria with a few remains were found. On the other hand, envelop of chloroplast broke and even completely destructed in serious abnormal chloroplast. Thylakoid and grana lamella of chloroplast disappeared and stroma exported. In addition, envelop of chloroplast and stroma lamella in bundle-sheath cells were destructed and the starch granules accumulated and expanded. The abnormal nucleus was found and envelop of nucleus split. The chromatin distributed asymmetrically and degraded.