CAJ | 학술논문

[目的]明确砀山酥梨果实的生长发育与其果肉中内源激素含量的关系[方法]以砀山酥梨为试材,利用酶联免疫吸附分析法对砀山酥梨不同发育时期果肉内源激素变化动态进行了测定分析。[结果]生长促进型激素ZR、GA和IAA含量在砀山酥梨授粉后1 -15 d呈上升趋势,较高ZR、GA和IAA含量能促进砀山酥梨子房发育和正常坐果。果实中ZR含量在砀山酥梨第一个快速生长期开始时最高,之后呈下降趋势,直到果实成熟。IAA和GA的变化动态大致相似,在果实第一个快速生长期含量较高,随着果实发育逐渐减少,进入果实第二个快速生长期又迅速增加并达到最大值,与果实生长动态基本吻合。ABA含量分别在授粉后15、45、120 d出现高峰,与砀山酥梨生理落果和果实成熟时期基本一致。[结论]砀山酥梨果实的生长发育各时期持续时间及其内源激素含量也不相同,果实的生长发育与其内源激素的含量有密切关系。
[목적]명학탕산소리과실적생장발육여기과육중내원격소함량적관계[방법]이탕산소리위시재,이용매련면역흡부분석법대탕산소리불동발육시기과육내원격소변화동태진행료측정분석。[결과]생장촉진형격소ZR、GA화IAA함량재탕산소리수분후1 -15 d정상승추세,교고ZR、GA화IAA함량능촉진탕산소리자방발육화정상좌과。과실중ZR함량재탕산소리제일개쾌속생장기개시시최고,지후정하강추세,직도과실성숙。IAA화GA적변화동태대치상사,재과실제일개쾌속생장기함량교고,수착과실발육축점감소,진입과실제이개쾌속생장기우신속증가병체도최대치,여과실생장동태기본문합。ABA함량분별재수분후15、45、120 d출현고봉,여탕산소리생리낙과화과실성숙시기기본일치。[결론]탕산소리과실적생장발육각시기지속시간급기내원격소함량야불상동,과실적생장발육여기내원격소적함량유밀절관계。
[Objective] The aim was to clear the relationship between fruit growth and development and endogenous hormones content in the pulp of Dangshansu pear.[Method] Choosing Dangshansu pear as material,the dynamic changes of endogenous hormones in pulp during various development stages were determined by means of enzyme linked immunosorbent assay（ELISA）.[Result] The results indicated that the content of endogenous hormones like ZR,GA and IAA promoting fruit growth showed increase trend within 15 d after pollination,and higher content of ZR,GA and IAA could promote ovary development and normal fruit setting of Dangshansu pear.The content of ZR in pulp had the highest value at the beginning of the first fast growth period,and then showed decrease trend until fruit became mature.The dynamic changes of IAA and GA were similar,namely their contents were higher in the first fast growth period,and decreased with fruit development,and then increased rapidly and was up to the maximum value in the second fast growth period,corresponding with the growth dynamic of fruit.The peaks of ABA content could be found on the 15th,45th and 120th day after pollination,which corresponded with the physiological fruit dropping and maturing period of Dangshansu pear.[Conclusion] The duration of various development periods and content of endogenous hormones of Dangshansu pear were different,and fruit growth and development were closely related to the content of endogenous hormones.