河北北方学院学报：自然科学版
河北北方學院學報：自然科學版
하북북방학원학보：자연과학판
Journa of Hebei North University:Natural Science Edition
2011年
5期
31-33
,共3页
油菜蜂花粉%多糖%黄酮%清除亚硝酸盐
油菜蜂花粉%多糖%黃酮%清除亞硝痠鹽
유채봉화분%다당%황동%청제아초산염
bee pollen%polysaccharide%flavonoids%elimination of nitrite
目的研究油菜蜂花粉中多糖和乙醇提取物对亚硝酸盐的清除作用.方法采用水提醇沉法对油菜蜂花粉中多糖进行提取,采用超声法制备乙醇提取物.并用紫外分光光度法测定多糖和乙醇提取物中黄酮的含量及其对亚硝酸盐的清除作用.结果测定油菜蜂花粉中黄酮含量为2.43%.多糖含量为10.79%.花粉乙醇提取物浓度为2.333mg/ml时对亚硝酸钠的清除率最大,清除率为25.37%.花粉多糖浓度为4.317mg/ml时对亚硝酸钠的清除作用最大,清除率为41.70%.结论坝上油菜蜂花粉乙醇提取物和多糖对亚硝酸盐有较高的清除作用.
目的研究油菜蜂花粉中多糖和乙醇提取物對亞硝痠鹽的清除作用.方法採用水提醇沉法對油菜蜂花粉中多糖進行提取,採用超聲法製備乙醇提取物.併用紫外分光光度法測定多糖和乙醇提取物中黃酮的含量及其對亞硝痠鹽的清除作用.結果測定油菜蜂花粉中黃酮含量為2.43%.多糖含量為10.79%.花粉乙醇提取物濃度為2.333mg/ml時對亞硝痠鈉的清除率最大,清除率為25.37%.花粉多糖濃度為4.317mg/ml時對亞硝痠鈉的清除作用最大,清除率為41.70%.結論壩上油菜蜂花粉乙醇提取物和多糖對亞硝痠鹽有較高的清除作用.
목적연구유채봉화분중다당화을순제취물대아초산염적청제작용.방법채용수제순침법대유채봉화분중다당진행제취,채용초성법제비을순제취물.병용자외분광광도법측정다당화을순제취물중황동적함량급기대아초산염적청제작용.결과측정유채봉화분중황동함량위2.43%.다당함량위10.79%.화분을순제취물농도위2.333mg/ml시대아초산납적청제솔최대,청제솔위25.37%.화분다당농도위4.317mg/ml시대아초산납적청제작용최대,청제솔위41.70%.결론패상유채봉화분을순제취물화다당대아초산염유교고적청제작용.
Objective To study nitrite scavenging effect of bee pollen polysaccharide and Ethanol extract. Methods Polysaccharide was extracted by the method of water alcohol extraction（WAE）,and the content and the clearance rate to Nitrite were determined by the UV-Spectrometer.The Ethanol extract was prepared by microwave extraction,and the content of flavonoids in it and the clearance rate to nitrite were determined by ultrasonic wave.Results The content of polysaccharide in bee pollen was 2.43%,the content of flavonoids in bee pollen 10.79%.The maximum scavenging rate of polysaccharide to nitrite was 41.7% with the content of 4.317 mg/ml,the maximum scavenging rate of Ethanol extract to nitrite was 25.37% with the content of 2.333 mg/ml.Conclusion The Ethanol extract and polysaccharide of Bashang Bee pollen have higher nitrite scavenging effect.
