CAJ | 학술논문

通直型巨龙竹Dendrocalamus sinicus是云南重要的经济竹种资源之一.为了保护和开发通直型巨龙竹种质资源,应用简单序列重复区间扩增(ISSR)标记对通直型巨龙竹核心分布区不同地理种源的遗传变异进行了研究.从80个引物中筛选出7个用于正式扩增,在所研究的4个居群共54个个体中检测到54个多态位点.在居群水平上,多态位点百分率为9.59%,Nei's基因多样性指数(H)争Shannon信息指数(I)分别为0.036 3和0.053 6;在物种水平上,多态位点百分率为73.97%.H和I分别为0.260 0和0.3921.居群间的遗传分化系数(G_(st))达0.863 4,显示不同居群间遗传分化很大.造成通直型巨龙竹不同地理种源遗传分化的主要原因可能是生境片断化和自然条件下,花而不实造成的种子传播困难.
통직형거룡죽Dendrocalamus sinicus시운남중요적경제죽충자원지일.위료보호화개발통직형거룡죽충질자원,응용간단서렬중복구간확증(ISSR)표기대통직형거룡죽핵심분포구불동지리충원적유전변이진행료연구.종80개인물중사선출7개용우정식확증,재소연구적4개거군공54개개체중검측도54개다태위점.재거군수평상,다태위점백분솔위9.59%,Nei's기인다양성지수(H)쟁Shannon신식지수(I)분별위0.036 3화0.053 6;재물충수평상,다태위점백분솔위73.97%.H화I분별위0.260 0화0.3921.거군간적유전분화계수(G_(st))체0.863 4,현시불동거군간유전분화흔대.조성통직형거룡죽불동지리충원유전분화적주요원인가능시생경편단화화자연조건하,화이불실조성적충자전파곤난.
The straight-culmed type of Dendrocalamus sinicus is one of the most important economic bam-boo resources in Yunnan Province, China. To protect and develop native germplasm resources of the straight-culmed D. Sinicus in Yunnan, the genetic diversity of four straight-culmed provenances of D. Sini-cus was analyzed based on inter-simple sequence repeat(ISSR) markers for percentage of polymorphic bands (PPB), Nei's gent diversity(H), Shannon's information index(I) and Nei's coefficient of genetic differ-entiation(G_(st)). Results showed seven informative and reliable primers with 54 polymorphic loci that were cho-sen from 80 pre-screened primers. At the population level, PPB was 9.59%, H was 0.036 3, and I was 0.053 6; whereas at the species level, PPB was 73.97%, H was 0.260 0 and I was 0.3921. Also, G, was 0.863 4. G_(st) indicated a high degree of genetic differentiation among straight-culmed D. Sinicus provenances most likely due to habitat fragmentation and flowering without seed.