CAJ | 학술논문

建立了HPLC-UV法检测土壤中多环芳烃类物质3-硝基苯并蒽酮的方法.采用on UltimateXB-C18（5μm,150mm×4.6mm i.d.）柱,流动相为甲醇∶水=7∶3（v/v）,流速为0.5mL/min,柱温为30℃,检测波长为391nm.在此色谱条件下,3-硝基苯并蒽酮在0.2～40μg/mL范围内具有良好的线性关系,最低浓度检测限为78ng/mL（S/N=3）.利用超声波萃法对土壤中的3-硝基苯并蒽酮进行了萃取,并用HPLC-UV法测出了其含量.
건립료HPLC-UV법검측토양중다배방경류물질3-초기분병은동적방법.채용on UltimateXB-C18（5μm,150mm×4.6mm i.d.）주,류동상위갑순∶수=7∶3（v/v）,류속위0.5mL/min,주온위30℃,검측파장위391nm.재차색보조건하,3-초기분병은동재0.2～40μg/mL범위내구유량호적선성관계,최저농도검측한위78ng/mL（S/N=3）.이용초성파췌법대토양중적3-초기분병은동진행료췌취,병용HPLC-UV법측출료기함량.
A HPLC-UV method for the determination of 3-nitrobenzanthrone（3-NBA） in surface soil was developed.HPLC analysis was performed on Ultimate XB-C18（5μm,150mm×4.6mm i.d.） with MeOH-water as the mobile phase.The flow rate kept at 0.5mL/min,the column temperature was set to 30℃ and the detection wavelength was 391nm.The linear range is 0.2-40μg/mL for 3-NBA.The detection limit is 7.8ng/mL（S/N=3）.Using ultrasonic extraction,3-NBA in soil samples were extracted,and tested the content of 3-NBA by HPLC-UV.