CAJ | 학술논문

目的:探讨胎膜组织骨形成蛋白-2(BNP-2)及基质金属蛋白酶-9(MMP-9)与胎膜早破(PROM)发生的关系.方法:选择足月临产产妇、足月PROM产妇、早产临产产妇、早产PROM产妇及足月未临产产妇(对照组)各18例,分娩后取破口处胎膜组织,采用免疫组织化学法染色,并使用病理影像多媒体图文操作系统对BMP-2、MMP-9阳性表达物的面积积分光密度(AIOD)进行半定量分析.结果:①胎膜组织中BMP-2和MMP-9阳性表达的AIOD在足月临产组、足月PRON组、早产临产组、早产PROM组均高于对照组,差异均有统计学意义(P<0.0071);②足月PROM组BMP-2和MMP-9高于足月临产组(P=0.0065,P=0.0069);早产PROM组BNP-2和NMP-9高于早产临产组(P=0.0069,P=0.0052);早产PROM组BMP-2和MMP-9高于足月PROM组(P=0.0037,P=0.0065);③5组胎膜组织中BMP-2和MMP-9的表达均呈明显正相关(r_s=0.76159,P<0.0001).结论:BMP-2和MMP-9与胎膜破裂的发生密切相关,胎膜组织中BMP-2与MMP-9的表达水平相关.
목적:탐토태막조직골형성단백-2(BNP-2)급기질금속단백매-9(MMP-9)여태막조파(PROM)발생적관계.방법:선택족월임산산부、족월PROM산부、조산임산산부、조산PROM산부급족월미임산산부(대조조)각18례,분면후취파구처태막조직,채용면역조직화학법염색,병사용병리영상다매체도문조작계통대BMP-2、MMP-9양성표체물적면적적분광밀도(AIOD)진행반정량분석.결과:①태막조직중BMP-2화MMP-9양성표체적AIOD재족월임산조、족월PRON조、조산임산조、조산PROM조균고우대조조,차이균유통계학의의(P<0.0071);②족월PROM조BMP-2화MMP-9고우족월임산조(P=0.0065,P=0.0069);조산PROM조BNP-2화NMP-9고우조산임산조(P=0.0069,P=0.0052);조산PROM조BMP-2화MMP-9고우족월PROM조(P=0.0037,P=0.0065);③5조태막조직중BMP-2화MMP-9적표체균정명현정상관(r_s=0.76159,P<0.0001).결론:BMP-2화MMP-9여태막파렬적발생밀절상관,태막조직중BMP-2여MMP-9적표체수평상관.
Objective:To study the correlation between the bone morphogenetic proteins (BMP-2) and matrix metalloproteinase-9(MMP-9) in fetal membrane and premature rupture of membranes. Methods: 18 samples were selected separately from each of the following groups: puerpera with term pregnancy in labor, puerpera with term premature rupture of membranes(PROM), puerpera in preterm labor, puerpera with pre-tenm PROM and puerpera with term pregnancy not in labor (control group). After labor, fetal membranes in the rupture site were taken out. Immunobistochemistry and pathologic image multimedia operation system were adopted to semiquantitative analyze the area integrated optical density (AIOD) of the positive expression of BMP-2 and MMP-9. Results:①The AIOD of BMP-2 and MMP-9 positive expression in fetal membranes was higher in term pregnancy in labor group, term PROM group, preterm labor group and PROM group than that in control group. There was statistic difference ( P < 0. 0071). ②The AIOD of BMP-2 and MMP-9 in term PROM group was higher than that in term pregnancy in labor group(P=0.0065,P=0.0069), which was higher in PROM group than that in preterm labor group (P=0.0069, P = 0.0052) and term PROM group P=0.0037, P =0.0065),③There was obviously positive correlation between BMP-2 and MMP-9 expression in the fetal membranes in five groups. ( r_s =0.76159,P<0.0001). Conclusions: The expression of MMP-9 and BMP-2 is closely relevant to the repture of membranes. There is a correlation between BMP-2 and MMP-9 expression in fetal membranes.