CAJ | 학술논문

[目的]探讨3个紫薇品种的花粉离体培养与萌发.[方法]以3个紫薇品种('红蝶飞舞'、'翠盘7薇'和'紫爪银薇')的盛花期花粉为材料,对花粉离体培养及花粉管萌发进行了研究.[结果]筛选出紫薇花粉萌发的最适培养基为150g/L蔗糖+60mg/L硼酸+20wg/L CaCI2+20 mg/L MgS04+100 mg/L KNO,其中蔗糖和硼酸的浓度对紫薇花粉萌发有明显影响.紫薇的长、短花丝萌发力存在差异,其中'紫爪银薇'和'红蝶飞舞'短花丝的花粉萌发力高于长花丝,而'紫爪银薇'相反.在荧光显微镜下观察,自交授粉后2～6 h内化粉萌发形成花粉管,花粉管生长速度由快到慢.[结论]为培育紫薇新品种提供了理论依据.
[목적]탐토3개자미품충적화분리체배양여맹발.[방법]이3개자미품충('홍접비무'、'취반7미'화'자조은미')적성화기화분위재료,대화분리체배양급화분관맹발진행료연구.[결과]사선출자미화분맹발적최괄배양기위150g/L자당+60mg/L붕산+20wg/L CaCI2+20 mg/L MgS04+100 mg/L KNO,기중자당화붕산적농도대자미화분맹발유명현영향.자미적장、단화사맹발력존재차이,기중'자조은미'화'홍접비무'단화사적화분맹발력고우장화사,이'자조은미'상반.재형광현미경하관찰,자교수분후2～6 h내화분맹발형성화분관,화분관생장속도유쾌도만.[결론]위배육자미신품충제공료이론의거.
[Objective] The research aimed at investigating the in vitro culture and germination of three cultivars of Lagerstroemia indica L.[Method] The pollen of three L. indica cultivars Hongdiefeiwu, Cuipanjinwei and Zizhuayinwei in full flowering stage was collected as materials to study the effects of in vitro culture on pollen viability and germination. [Result] The optimum medium for L. indica pollen germination was 150 g/L sucrose + 60 mg/L boric acid + 20 mg/L CaCl2 +20 mg/L MgSO4 +100 ng/L KNO3, in which, the concentrations of sucrose and boric acid could significantly influence the pollen germination. In addition, the vitality of pollen at long filament differed from that at short filament,wherein, the vitality of pollen at short filament of Zizhuayinwei and Hongdiefeiwu was higher than that at their long filament, while Cuipanjinwei showed the opposite result. Within 2 -6 h after pollination self, pollen germinated to form pollen tube, which grew fast firstly and then slowly.[Conclusion] The study provided theoretical reference for breeding new L, indica cultivar.