CAJ | 학술논문

目的：检测大、小鼠空肠、肝及肾组织中P型铜转运ATP酶（ATP7B）蛋白的表达。方法：分别取6只SD大鼠和7只昆明小鼠,取空肠、肝及肾组织,常规石蜡切片,采用免疫组化法检测ATP7B蛋白的表达。结果：大、小鼠空肠绒毛上皮的纹状缘和近基底部、小肠腺、肾小管和肝细胞中均有不同程度的ATP7B蛋白表达。大鼠空肠、肝及肾组织ATP7B的积分光密度分别为（224474.380±228536.513）、（94116.484±45821.113）和（118128.741±41046.629）,小鼠上述3种组织中ATP7B的积分光密度分别为（129023.602±87570.591）、（150614.511±52981.792）和（118403.634±53656.681）,大、小鼠同一组织器官ATP7B的积分光密度相比,差异均无统计学意义（t=1.239、1.355、0.009,P均〉0.05）。结论：ATP7B蛋白在大、小鼠空肠、肝及肾组织中均有表达,其表达量的高低与动物种属关系不大。
목적：검측대、소서공장、간급신조직중P형동전운ATP매（ATP7B）단백적표체。방법：분별취6지SD대서화7지곤명소서,취공장、간급신조직,상규석사절편,채용면역조화법검측ATP7B단백적표체。결과：대、소서공장융모상피적문상연화근기저부、소장선、신소관화간세포중균유불동정도적ATP7B단백표체。대서공장、간급신조직ATP7B적적분광밀도분별위（224474.380±228536.513）、（94116.484±45821.113）화（118128.741±41046.629）,소서상술3충조직중ATP7B적적분광밀도분별위（129023.602±87570.591）、（150614.511±52981.792）화（118403.634±53656.681）,대、소서동일조직기관ATP7B적적분광밀도상비,차이균무통계학의의（t=1.239、1.355、0.009,P균〉0.05）。결론：ATP7B단백재대、소서공장、간급신조직중균유표체,기표체량적고저여동물충속관계불대。
Aim：To detect the expression of ATP7B in jejunum, liver and kidney tissue of rats and mice.Methods：The jejunum, liver and kidney tissue was excised from 6 SD rats and 7 Kunming mice. The expression of ATP7B was detected using immunohistochemical staining.Results：ATP7B positive immunohistochemical staining was localized in striated border,basilar part of epithelial cells and small intestine glands of jejunum, in the renal tubules for the kidney tissue,and in hepatic cells for the liver tissue,however, the expression levels were different. The integral optical density of ATP7B in rat jejunum, liver and kidney tissue was （224 474.380±228 536.513）,（94 116.484±45 821.113）,（118 128.741±41 046.629）,which in the above tissue of mice was （129 023.602±87 570.591）,（150 614.511±52 981.792）,（118 403.634±53 656.681）,and there was no significant difference in the expression levels of ATP7B between the same tissue of rats and mice（t=1.239,1.355,and 0.009,P0.05）.Conclusion： The expression of ATP7B is found in jejunum, liver and kidney tissue of rat and mouse, but the level of ATP7B expression is not related with the animal genus.