江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2010年
1期
119-122
,共4页
谷晓霞%刘建华%胡功政%苑丽%潘玉善%王磊%陈玉霞
穀曉霞%劉建華%鬍功政%苑麗%潘玉善%王磊%陳玉霞
곡효하%류건화%호공정%원려%반옥선%왕뢰%진옥하
鸭大肠杆菌%耐酶抑制剂β-内酰胺酶%纸片扩散法%耐药性
鴨大腸桿菌%耐酶抑製劑β-內酰胺酶%紙片擴散法%耐藥性
압대장간균%내매억제제β-내선알매%지편확산법%내약성
duck E. coli%IRBLs%K-B test%drug-resistance
根据耐酶抑制剂β-内酰胺酶(IRBLs)的耐药表型特点,应用纸片扩散法(K-B法)检测40株鸭大肠杆菌产IRBLs情况并分析产IRBLs菌的耐药性.结果表明:产IRBLs阳性株4株,阳性率为10%,其对阿莫西林、阿莫西林/棒酸、氨苄西林、氨苄西林/舒巴坦、替卡西林/棒酸、氟苯尼考、庆大霉素、新霉素、多西环素耐药,说明IRBLs产生菌已在我国兽医临床上出现,且呈现多重耐药的特点.此外,2株产IRBLs菌对哌拉西林/三唑巴坦敏感,表明三唑巴坦对哌拉西林有一定的保护作用.再者,1株产IRBLs菌对三代头孢耐药,该菌株可能同时产生超广谱β-内酰胺酶(ESBLs)或AmpC酶,这提示IRBLs发展的新趋势.
根據耐酶抑製劑β-內酰胺酶(IRBLs)的耐藥錶型特點,應用紙片擴散法(K-B法)檢測40株鴨大腸桿菌產IRBLs情況併分析產IRBLs菌的耐藥性.結果錶明:產IRBLs暘性株4株,暘性率為10%,其對阿莫西林、阿莫西林/棒痠、氨芐西林、氨芐西林/舒巴坦、替卡西林/棒痠、氟苯尼攷、慶大黴素、新黴素、多西環素耐藥,說明IRBLs產生菌已在我國獸醫臨床上齣現,且呈現多重耐藥的特點.此外,2株產IRBLs菌對哌拉西林/三唑巴坦敏感,錶明三唑巴坦對哌拉西林有一定的保護作用.再者,1株產IRBLs菌對三代頭孢耐藥,該菌株可能同時產生超廣譜β-內酰胺酶(ESBLs)或AmpC酶,這提示IRBLs髮展的新趨勢.
근거내매억제제β-내선알매(IRBLs)적내약표형특점,응용지편확산법(K-B법)검측40주압대장간균산IRBLs정황병분석산IRBLs균적내약성.결과표명:산IRBLs양성주4주,양성솔위10%,기대아막서림、아막서림/봉산、안변서림、안변서림/서파탄、체잡서림/봉산、불분니고、경대매소、신매소、다서배소내약,설명IRBLs산생균이재아국수의림상상출현,차정현다중내약적특점.차외,2주산IRBLs균대고랍서림/삼서파탄민감,표명삼서파탄대고랍서림유일정적보호작용.재자,1주산IRBLs균대삼대두포내약,해균주가능동시산생초엄보β-내선알매(ESBLs)혹AmpC매,저제시IRBLs발전적신추세.
Based on the drug-resistance characters of IRBLs,we studied the prevalence of IRBLs among 40 clinical isolates in duck E.coli was studied by K-B test and the drug-resistance of IRBLs-producer to antibacterial drugs was analyzed.The results showed that 4 isolates produced IRBLs,with resistance to amoxicillin,amoxicillin/clavulanic acid,ampicillin,ampicillin/sulbactam,ticarcillin/clavulanicacid,florfenicol,gentamycin,neomycin,sulfadiazine and doxycycline,which suggested that IRBLs-producing isolates had emerged in China,and were characterized by multidrug-resistance.Besides,two IRBLs-producing strains were susceptible to piperacillin/tazobactam,resulting from the protection of tazobactam to piperacillin.What's more,there was one IRBLs-producing strain which was resistant to the third generation cephalosporins,which explained it may also produced ESBLs or AmpC,and it implyed the further development trend about IRBLs.
